Template:Current week ecosystem agenda: Difference between revisions

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2020 Week 30
{{Template:Science for Ukraine}}


* '''Jul-20 Mo'''  
2024 Week 20
 
* '''May-13 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
|'''How can you compare respirometric experiments using an internal normalization?'''<br>
|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”.  
Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.  
<br>
<br>
- »[[Flux control ratio]]«
- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
 
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]«  
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* '''Jul-21 Tu'''
* '''May-14 Tu'''
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* '''May-15 We'''
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* '''May-16 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br>
|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]«  
- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print].  [[Lantier 2020 Diabetes|»Bioblast link«]]
- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
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* '''Jul-22 We'''  
* '''May-17 Fr'''
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'''<big>Next Week</big>'''


2024 Week 21


* '''May-20 Mo'''
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
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* '''May-21 Tu'''
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* '''Jul-23 Th'''  
* '''May-22 We''' - International Day for Biological Diversity
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? '''  
|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br>
* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«
:from the O2k-Network »[[NO Tromsoe Wood SH]]«
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* '''Jul-24 Fr'''  
* '''May-23 Th'''
 
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* '''May-24 Fr'''
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* '''Jul-25 Sa'''

Revision as of 09:04, 13 May 2024

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2024 Week 20
  • May-13 Mo
O2k-Open SupportInfo: take care Do you see fluctuations of O2 slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O2 slope negative.
- »Fluctuations of O2 slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«


  • May-14 Tu

  • May-15 We

  • May-16 Th
O2k-Open SupportInfo: improve "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«


  • May-17 Fr

Next Week

2024 Week 21
  • May-20 Mo
MitoPedia
How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test« 
- communicated by »Baglivo Eleonora« and »Schmitt Sabine« 


  • May-21 Tu

  • May-22 We - International Day for Biological Diversity
O2k-Publications in the MiPMap "We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."
  • Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«
from the O2k-Network »NO Tromsoe Wood SH«

  • May-23 Th

  • May-24 Fr

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