Template:Current week ecosystem agenda: Difference between revisions

Revision as of 09:04, 13 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

Next Week

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

@@ Line 1: / Line 1: @@
-Week 43
+{{Template:Science for Ukraine}}
-* '''Oct-19 Mo'''
+Week 20
+* '''May-13 Mo'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
+<br>
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
+|}
+----
+* '''May-14 Tu'''
+----
+* '''May-15 We'''
+----
+* '''May-16 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?'''<br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.<br>
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
--  You can read more about it in the following publication: »[[Krumschnabel 2014 Methods Enzymol]]« <br>
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
 |}
 ----
-* '''Oct-20 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]
+* '''May-17 Fr'''
+----
+'''<big>Next Week</big>'''
+Week 21
+* '''May-20 Mo'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
+----
+* '''May-21 Tu'''
 ----
-* '''Oct-21 We'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies '''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-:from the O2k-Network »[[US NC Winston-Salem Molina AJA]]«
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-*Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.[[Tyrrell 2016 Redox Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
-|}
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Human [[PBMC]] and [[Platelet]] - minimally invasive samples for HRR '''<br>
-Read about isolation procedures for these sample types.<br>
-- »'''[[MiPNet21.17_BloodCellsIsolation|MiPNet21.17 Blood Cells Isolation]]'''«  <br>
-- communicated by »[[Cecatto Cristiane]]« and »[[Garcia-Souza Luiz F]]«
 |}
 ----
-* '''Oct-22 Th'''
+* '''May-23 Th'''
-:{|
+----
+* '''May-24 Fr'''
-| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
-|''' LEAK respiration relates to intrinsic uncoupling.   '''<br>
-Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.  <br>
-- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''« - Table 2. Terms on respiratory coupling and uncoupling  <br>
-- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]« and »[[Gnaiger Erich]]«
-|}
 ----
-* '''Oct-23 Fr'''
-:{|
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
-|'''Simultaneous measurement of O<sub>2</sub> flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system''' <br>
-: from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[AT Innsbruck Gnaiger E]]«<br>
-* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.   [[Krumschnabel 2014 Methods Enzymol|»Bioblast link«]]
-|}