Template:Current week ecosystem agenda: Difference between revisions

Revision as of 09:04, 13 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

Next Week

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 41
-*'''Oct- 09 Mo'''
+Week 20
-----
-*'''Oct- 10 Tu'''
+* '''May-13 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''What is the best fluorophore and concentration thereof for measuring mt-membrane potential?'''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-It depends on sample characteristics -  test the concentration of different fluorophores (safranin, TMRM and rhodamine 123) with your specific model to evaluate for inhibitory or uncoupling effects on respiration. <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[https://wiki.oroboros.at/index.php/Mitochondrial_membrane_potential#Different_methods_for_measurements_of_mt-membrane_potential mt-membrane potential measurement]'''« <br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-*'''Oct- 11 We'''
+* '''May-14 Tu'''
-:{|
+----
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+* '''May-15 We'''
-| The abbreviation 'Dith' has been used previously for '''Dithionite''' and is stepwise replaced by the shorter 'Dit'. '''<br>
-- »'''[https://wiki.oroboros.at/index.php/Dithionite Dithionite, Dit]'''«
-|}
 ----
-*'''Oct- 12 Th''' - World Arthritis Day
+* '''May-16 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''“Mitochondrial dysfunction did not seem to be a major contributing factor for insulin resistance in RA [rheumatoid arthritis]”''' <br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-* Moellering DR, Smith-Johnston K, Kelley C, Sammy MJ, Benedict J, Brock G, Johnson J, Baskin KK, Jarjour WN, Belury MA, Reiser PJ, Nagareddy PR, Hanaoka BY (2023) Association between skeletal muscle mitochondrial dysfunction and insulin resistance in patients with rheumatoid arthritis: a case-control study. [[Moellering 2023 Arthritis Res Ther|»Bioblast link«]]
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-:from the O2k-Network »[[US AL Birmingham Moellering_DR]]«
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Oct- 13 Fr'''
+* '''May-17 Fr'''
 ----
+'''<big>Next Week</big>'''
-'''<big>Next Week</big>
+Week 21
-'''
-Week 42
-*'''Oct- 16 Mo'''
+* '''May-20 Mo'''
-:::: '''»[[2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT]]«'''
-----
-*'''Oct- 17 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Noticing higher values of J°1 when using the O2k sV-chamber (0.5 mL) compared with the 2mL-chamber? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-The instrumental oxygen background is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- »'''[[O2k-sV-Module|O2k-sV-Module]]'''«, »'''[[MiPNet06.03 POS-calibration-SOP|POS calibration-SOP]]'''«<br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
+|}
-|}
 ----
-*'''Oct- 18 We'''
+* '''May-21 Tu'''
 ----
-*'''Oct- 19 Th'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"MDH2 is a metabolic switch rewiring [electron transfer system] and TCA fuelling"''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Molinié T, Cougouilles E, David C, Cahoreau E, Portais JC, Mourier A (2022) MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. [[Molinie 2022 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[FR Bordeaux Mourier A]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
+----
+* '''May-23 Th'''
 ----
-*'''Oct- 20 Fr'''
+* '''May-24 Fr'''
 ----