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Latest revision as of 08:52, 27 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

Next Week

2024 Week 23

Jun-03 Mo

Jun-04 Tu

Jun-05 We

Jun-06 Th

"Silencing of mt-tRF-Leu^TAA in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."

Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. »Bioblast link«

from the O2k-Network »CH Lausanne Place N«

Jun-07 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 06
-* '''Feb-05 Mo'''
+Week 22
+* '''May-27 Mo'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."''' <br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-* Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. [[Palacka 2021 Int J Mol Sci|»Bioblast link«]]
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-:from the O2k-Network »[[SK Bratislava Sumbalova Z]]«
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Feb-06 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Feb-07 We'''
+* '''May-29 We'''
 ----
-* '''Feb-08 Th'''
+* '''May-30 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Can I use the same stock solutions for all reagents for different O2k-chamber volumes?'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-"For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration."<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
--»'''[[MiPNet09.12 O2k-Titrations]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Feb-09 Fr'''
+* '''May-31 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 07
+Week 23
-* '''Feb-12 Mo'''
+* '''Jun-03 Mo'''
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' What is the O<sub>2</sub> solubility factor of the medium (''F''<sub>M</sub>) and why is it important? '''<br>
-''F''<sub>M</sub> expresses the effect of the salt concentration on oxygen solubility relative to pure water, and varies depending on the temperature and composition of the medium. The correct ''F''<sub>M</sub> needs to be entered in DatLab when performing an air calibration. <br>
-- »'''[[Oxygen solubility factor]]'''« , »'''[[Oxygen_calibration_-_DatLab#Air_calibration_in_DatLab|Air calibration in DatLab]]'''« <br>
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
-|}
 ----
-* '''Feb-13 Tu'''
+* '''Jun-04 Tu'''
 ----
-* '''Feb-14 We'''
+* '''Jun-05 We'''
 ----
-* '''Feb-15 Th''' - International Childhood Cancer Day
+* '''Jun-06 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Deleterious mtDNA mutations contribute significantly to the tumorigenesis of pediatric cancers"''' <br>
+|'''"Silencing of mt-tRF-Leu<sup>TAA</sup> in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."''' <br>
-* Triska P, Kaneva K, Merkurjev D, Sohail N, Falk MJ, Triche TJ Jr, Biegel JA, Gai X (2019) Landscape of germline and somatic mitochondrial DNA mutations in pediatric malignancies. [[Triska 2019 Cancer Res|»Bioblast link«]]
+* Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. [[Jacovetti 2024 Mol Metab|»Bioblast link«]]
-:from the O2k-Network »[[US_PA_Philadelphia_Falk_MJ]]«
+:from the O2k-Network »[[CH Lausanne Place N]]«
 |}
 ----
-* '''Feb-16 Fr'''
+* '''Jun-07 Fr'''
 ----