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Latest revision as of 08:52, 27 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

Next Week

2024 Week 23

Jun-03 Mo

Jun-04 Tu

Jun-05 We

Jun-06 Th

"Silencing of mt-tRF-Leu^TAA in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."

Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. »Bioblast link«

from the O2k-Network »CH Lausanne Place N«

Jun-07 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 11
-* '''Mar-11 Mo'''
+Week 22
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered.   <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Mar-12 Tu'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
 ----
-* '''Mar-13 We'''
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:BEC-logo.png|100px|link=https://www.bioenergetics-communications.org/index.php/bec |Bioenergetics Communications]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''''Rewarded'' Open Access - compensating authors'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-Together with the MiP''society'', BEC is raising funds to reward the first 10 papers accepted for publication in BEC in 2024: 600 EUR to attend EBEC2024 and 2-year MiP''membership''.
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
--»[https://whydonate.com/en/fundraising/BEC Support open access in science]«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- »[https://www.bioenergetics-communications.org/index.php/bec/about/submissions Submit a manuscript]«
 |}
 ----
-* '''Mar-14 Th'''
+* '''May-31 Fr'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Mitochondrial ultrastructure and respiration are affected during EDL [''Extensor digitorum longus'' muscle] aging.''' <br>
-* Fernando R, Shindyapina AV, Ost M, Santesmasses D, Hu Y, Tyshkovskiy A, Yim SH, Weiss J, Gladyshev VN, Grune T, Castro JP (2023) Downregulation of mitochondrial metabolism is a driver for fast skeletal muscle loss during mouse aging.  [[Fernando 2023 Commun Biol|»Bioblast link«]]
-:from the O2k-Network »[[DE Leipzip Ost M]]«
-|}
 ----
-* '''Mar-15 Fr'''
-----
 '''<big>Next Week</big>'''
-Week 12
+Week 23
-* '''Mar-18 Mo'''
+* '''Jun-03 Mo'''
-::::: '''[[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' Mar 18-21
 ----
-* '''Mar-19 Tu'''
+* '''Jun-04 Tu'''
-:::: 15:05 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' - ''[[Donnelly_2024_EU-METAHEART_MC2_Meeting |Impact of hypoxia on mitochondrial function]]''
 ----
-* '''Mar-20 We'''
+* '''Jun-05 We'''
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' What if ''Alert: Error in titrations!'' appears in your O2 analysis template file?  '''<br>
-The O2 analysis template recognizes discrepancies between the protocol selected in the first tab and the imported marks. Check the titration sequence and mark names against the protocol copied from the DLD file.<br>
-- »'''[[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|O<sub>2</sub> flux analysis]]'''«<br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
-|}
 ----
-* '''Mar-21 Th'''
+* '''Jun-06 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' How well do you handle your Hamilton syringes? What is a cleaning cycle?'''<br>
+|'''"Silencing of mt-tRF-Leu<sup>TAA</sup> in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."''' <br>
-"A syringe cleaning cycle consists of filling an empty syringe with a washing solution while immersing [...] also the base of the barrel into the washing solution, without fully removing the plunger, and emptying the syringe into the waste beaker". <br>
+* Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. [[Jacovetti 2024 Mol Metab|»Bioblast link«]]
-- »'''[[MiPNet19.14 SOP Hamilton microsyringes]]'''«<br>
+:from the O2k-Network »[[CH Lausanne Place N]]«
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Mar-22 Fr'''
+* '''Jun-07 Fr'''
 ----