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| {{MitoPedia
| | #REDIRECT[[Complex IV]] |
| |abbr=CIV
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| |description='''CIV''': Electron flow through [[Complex IV]] (cytochrome ''c'' oxidase) is measured in intact mitochondria after inhibiton of CIII by antimycin A, and addition of [[ascorbate]] (As) and the artificial substrate [[TMPD]] (Tm). Ascorbate has to be titrated first. It reduces [[TMPD]], which further reduces cytochrome ''c'', which is the substrate of CIV. Since CIV is a proton pump of the electron transfer system, the single step of CIV-linked respiration can be measured in different coupling states: ''L'', ''P'', and ''E''. Measurement of CIV activity requires uncoupler titrations to eliminate any potential control by the phosphorylation system, and a cytochrome ''c'' test to avoid any limitation by cytochrome ''c'' release. Total oxygen uptake in the ascorbate&TMPD(&c) stimulated state (Tm) has to be corrected for chemical background oxygen consumption.
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| |info=[[Pathway control state]], [[Gnaiger 2002 Biochem Soc Trans]]
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| }}
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| {{MitoPedia concepts
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| |mitopedia concept=Respiratory state, SUIT state
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| }}
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| {{MitoPedia topics
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| |mitopedia topic=EAGLE
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| }}
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| Communicated by [[Gnaiger E]] 2016-01-20; edited 2016-11-07.
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| == CIV(L) ==
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| == CIV(P) ==
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| == CIV(E) ==
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| [[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]]
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| == Publications: Complex IV single step ==
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| '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
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| Default sorting: chronological. Empty fields appear first in ascending order.
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| {{#ask:[[Category:Publications]] [[Pathways::CIV]]
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| |?Was published in year=Year
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| |?Has title=Reference
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| |?Coupling states=Coupling
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| |?Pathways=Pathway
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| |?Preparation=Preparation
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| |?Mammal and model=Organism
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| |?Tissue and cell=Tissue;cell
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| |format=broadtable
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| |limit=5000
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| |offset=0
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| |sort=Was published in year
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| |order=descending
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| }}
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| [[Image:MiPMap Publication.jpg|left|120px|link=http://www.bioblast.at/index.php/MiPMap|Publications in the MiPMap]] | |
| == Abstracts: Complex IV single step ==
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| '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
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| {{#ask:[[Category:Abstracts]] [[Pathways::CIV]]
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| |?Was submitted in year=Year
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| |?Has title=Reference
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| |?Coupling states=Coupling
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| |?Pathways=Pathway
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| |?Preparation=Preparation
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| |?Mammal and model=Organism
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| |?Tissue and cell=Tissue;cell
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| |format=broadtable
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| |limit=5000
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| |offset=0
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| |sort=Was submitted in year
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| |order=descending
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| }}
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