Glutamate: Difference between revisions
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'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) weigh 1.871 g of L-Glutamic acid and transfer to 5 mL volumetric glass flask | ::1) weigh 1.871 g (or 1.691 g of anhydrous) L-Glutamic acid and transfer to 5 mL volumetric glass flask | ||
::2) add 3 ml H<sub>2</sub>Oย (pH = 6) | ::2) add 3 ml H<sub>2</sub>Oย (pH = 6) | ||
::3) neutralize with 5 N KOH (approx. 50 ยตl) | ::3) neutralize with 5 N KOH (approx. 50 ยตl) | ||
Revision as of 14:31, 28 July 2011
- high-resolution terminology - matching measurements at high-resolution
Glutamate
Description
Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix via glutamate dehydrogenase to ฮฑ-Ketoglutarate. Ammonia (the byproduct of the reaction) can pass freely through the mitochondrial membrane. See also Glutamate-aspartate carrier.
Abbreviation: G
Reference: MiPNet11.04; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
G: Glutamate (L-Glutamic acid, sodium salt, C5H8NO4Na; contains 1 mol/mol H2O); Sigma G 1626, 100 g, store at R.T.: FW = 187.1 or 169.1 anhydrous)
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 1.871 g (or 1.691 g of anhydrous) L-Glutamic acid and transfer to 5 mL volumetric glass flask
- 2) add 3 ml H2O (pH = 6)
- 3) neutralize with 5 N KOH (approx. 50 ยตl)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 5 ml and divide into 0.5 ml portions.
- 6) store frozen at -20 ยฐC
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 10 ยตl using a 25 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
