MiPNet10.05 O2-Concentration-Flux: Difference between revisions
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== Further Information== | == Further Information== | ||
:>> [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | :>> [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | ||
Revision as of 10:51, 2 December 2013
Gnaiger E (2005-2010) From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. - New edition: Gnaiger 2012 Chapter 1. |
Β» Open Access-MiPNet10.05.pdf; Versions
Abstract: O2k-Protocol for Oxygen flux In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the βrate of oxygen consumptionβ not expressed as a negative value? Why is the term βoxygen fluxβ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics.
Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption.
Eye-fitted slopes of oxygen chart recorder traces belong to the past. With DatLab, trends are resolved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication.
Reference
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
β’ Keywords: Archive
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP