MiPNet23.03 IOC128 Cambridge UK: Difference between revisions
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:::::: Cell respiration and H<sub>2</sub>O<sub>2</sub> production Β | :::::: Cell respiration and H<sub>2</sub>O<sub>2</sub> production Β | ||
== Programme == | |||
=== Monday May 07 === | |||
{| class="wikitable" | |||
|- class="hintergrundfarbe6" | |||
! Time | |||
! | |||
! Topic | |||
! Materials | |||
|- | |||
| 09:00-09:15 | |||
| | |||
|Arrival tea and coffee | |||
| | |||
|- | |||
| 09:15-10:15 | |||
| [[File:Gnaiger Erich.jpg|50px|left|Erich Gnaiger]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Lecture: '''Mitochondrial respiratory states and rates''' | |||
| | |||
|- | |||
| 10:15-11:00 | |||
| [[File:Gnaiger Erich.jpg|50px|left|Erich Gnaiger]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Lecture: '''Mitochondrial preparations: the versatility of O2k applications''' | |||
| | |||
|- | |||
| 11:00-11:15 | |||
|Β | |||
| Morning coffee break | |||
| | |||
|- | |||
| 11:15-12:15 | |||
| [[Image:Logo OROBOROS-DatLab.jpg|50px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Demo experiment: '''Getting started with the O2k and DatLab 7''' | |||
| | |||
|- | |||
| 12:15-13:00 | |||
| [[Image:Logo OROBOROS-DatLab.jpg|50px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Demo experiment: '''DatLab 7 innovations''' | |||
| | |||
|- | |||
| 13:00-14:00 | |||
| | |||
| Lunch break | |||
|- | |||
| 14:00-15:30 | |||
| [[Image:Logo OROBOROS-DatLab.jpg|50px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Lecture: '''Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling.''' | |||
| [[Doerrier_2018_Methods_Mol_Biol]] | |||
|- | |||
| 15:30-15:45 | |||
| | |||
| Tea break | |||
|- | |||
| 15:45-17:00 | |||
| [[File:Gnaiger Erich.jpg|50px|left|Erich Gnaiger]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Lecture: '''Normoxia β hyperoxia β hypoxia.''' | |||
| | |||
|} | |||
=== Tuesday May 08 === | |||
{| class="wikitable" | |||
|- class="hintergrundfarbe6" | |||
! Time | |||
! | |||
! Topic | |||
! Materials | |||
|- | |||
| 09:00-09:15 | |||
| | |||
|Arrival tea and coffee | |||
| | |||
|- | |||
| 09:15-09:45 | |||
| [[File:Gnaiger Erich.jpg|50px|left|Erich Gnaiger]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Lecture: '''O2 flux and H2O2 production: physiology and pathology''' | |||
| | |||
|- | |||
| 09:45-11:00 | |||
| [[Image:Logo OROBOROS-DatLab.jpg|50px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] | |||
| | |||
Hands-on: '''O2k calibration and quality control (O2 and H2O2)''' | |||
| | |||
|- | |||
| 11:00-11:15 | |||
|Β | |||
| Morning coffee break | |||
| | |||
|- | |||
| 11:15-13:00 | |||
| [[Image:Logo OROBOROS-DatLab.jpg|50px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] | |||
| | |||
Hands-on: '''O2k-FluoRespirometry for measurement of cell respiration and H2O2 production''' | |||
| | |||
|- | |||
| 13:00-14:00 | |||
| | |||
| Lunch break | |||
|- | |||
| 14:00-15:30 | |||
| [[Image:Logo OROBOROS-DatLab.jpg|50px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] | |||
| | |||
Hands-on: '''Data analysis, interpretation and discussion.''' | |||
| | |||
|- | |||
| 15:30-15:45 | |||
| | |||
| Tea break | |||
|- | |||
| 15:45-17:00 | |||
| [[File:Gnaiger Erich.jpg|50px|left|Erich Gnaiger]] | |||
| [[Gnaiger E |Erich Gnaiger]] | |||
Lecture: '''O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence).''' | |||
| | |||
|} | |||
== Preliminary list of participants== | == Preliminary list of participants== |
Revision as of 18:23, 2 May 2018
MiPNet23.03 IOC128 Cambridge UK
Cambridge UK, 2018 May 7-8. Oroboros satellite O2k-Workshop on High-Resolution FluoRespirometry IOC128. |
Oroboros (2018-05-07) Mitochondr Physiol Network
Abstract:
Oroboros satellite O2k-Workshop on High-Resolution FluoRespirometry in cooperation with UK Cambridge Murray AJ. Cambridge, United Kingdom, 2018 May 7.
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS, UK Cambridge Murray AJ
General information
- This O2k-Workshop takes place satellite to the Mitochondrial Medicine Conference in Hinxton, UK. Separate registration is required for this workshop.
Venue
- May 7
- The Graham Storey Room
- Trinity Hall - Cambridge
- Cambridge CB2 1TJ
- United Kingdom
- Venue
- May 8
- Department of Physiology, Development & Neuroscience
- University of Cambridge
Local organizers
Registration
- Download registration form: download pdf
- Limited number of participants.
Tutors and Lecturers
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
AndrΓ‘s MΓ©szΓ‘ros, MD., PhD., Chief Research Officer, Oroboros Instruments
Verena Laner, Chief operating officer (COO), Oroboros Instruments
Programme
- May 7 - full day: lectures and presentations
- May 8 - morning: lectures and presentations
- May 8 - afternoon: hands-on session
- Topics:
- Mitochondrial respiratory states and rates
- SUIT Protocols in DatLab 7
- Mitochondrial preparations: the versatility of O2k applications
- Topics:
- Normoxia β hyperoxia β hypoxia
- O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence)
- O2 flux and H2O2 production: physiology and pathology
- Experimental demo for
- 1. Getting started with the O2k and DatLab 7
- 2. DatLab 7 innovations
- Experimental demo for
- Hands-on (2018-05-08)
- O2k calibration and quality control (O2 and H2O2)
- Cell respiration and H2O2 production
- Hands-on (2018-05-08)
Programme
Monday May 07
Time | Topic | Materials | |
---|---|---|---|
09:00-09:15 | Arrival tea and coffee | ||
09:15-10:15 | Erich Gnaiger
Lecture: Mitochondrial respiratory states and rates |
||
10:15-11:00 | Erich Gnaiger
Lecture: Mitochondrial preparations: the versatility of O2k applications |
||
11:00-11:15 | Morning coffee break | ||
11:15-12:15 | Erich Gnaiger
Demo experiment: Getting started with the O2k and DatLab 7 |
||
12:15-13:00 | Erich Gnaiger
Demo experiment: DatLab 7 innovations |
||
13:00-14:00 | Lunch break | ||
14:00-15:30 | Erich Gnaiger
Lecture: Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling. |
Doerrier_2018_Methods_Mol_Biol | |
15:30-15:45 | Tea break | ||
15:45-17:00 | Erich Gnaiger
Lecture: Normoxia β hyperoxia β hypoxia. |
Tuesday May 08
Time | Topic | Materials | |
---|---|---|---|
09:00-09:15 | Arrival tea and coffee | ||
09:15-09:45 | Erich Gnaiger
Lecture: O2 flux and H2O2 production: physiology and pathology |
||
09:45-11:00 |
Hands-on: O2k calibration and quality control (O2 and H2O2) |
||
11:00-11:15 | Morning coffee break | ||
11:15-13:00 |
Hands-on: O2k-FluoRespirometry for measurement of cell respiration and H2O2 production |
||
13:00-14:00 | Lunch break | ||
14:00-15:30 |
Hands-on: Data analysis, interpretation and discussion. |
||
15:30-15:45 | Tea break | ||
15:45-17:00 | Erich Gnaiger
Lecture: O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence). |
Preliminary list of participants
Participant | Institution | |
---|---|---|
Davies Katie | University of Cambridge, UK | |
Fornaro Mara | Novartis Institutes for Biomedical Research, CH | |
Garrud Tessa | University of Cambridge, UK | |
Kapoor Rishab | Vanderbilt University Medical Center, USA | |
Knapp-Wilson Amber | University of Bristol , UK | |
Krause Fynn Nidas | University of Cambridge, UK | |
Martin Daniel | UK London Martin DS - University College London, UK | |
Sowton Alice | UK Cambridge Murray AJ - Trinity Hall, UK | |
Stanzani Giacomo | UK London Duchen MR - University College London, UK | |
Stevens Jia Liu | UK London Martin DS - University College London, UK | |
Tong Wen | University of Cambridge, UK | |
Yong Hannah | UK Cambridge Murray AJ - University of Cambridge, UK |
Mitochondrial Medicine Conference Hinxton
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- SchΓΆpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2018, Next
O2k-Workshops are listed as MitoGlobal Events.