MitoEAGLE data: blood and cultured cells: Difference between revisions
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Revision as of 14:58, 27 April 2016
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells β as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) β has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Torres-Quesada Omar Austria Innsbruck Doerrier Carolina Austria Innsbruck Fischer Christine Austria Innsbruck Hoppel Florian Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Gnaiger Erich Austria Innsbruck Karabatsiakis Alexander Austria Innsbruck Meszaros Andras T Belgium Namur Detraux Damien Belgium Liege Votion Dominique-Marie Belgium Namur Renard P Belgium Namur Arnould Thierry Belgium Leuven Bird Matthew Bosnia and Herzegovina Tuzla Jusic Amela Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil SΓ£o Paulo Crispim Marcell Brazil Sao Paulo Dominguez-Amorocho OA Brazil Sao Paulo Alencar Mayke Bezerra Croatia Zagreb Rubelj Ivica Czech Republic Vestec Zobalova Renata Czech Republic Pilsen Markova Michaela Czech Republic Pilsen Kripnerova M Czech Republic Prague Elkalaf Moustafa Czech Republic Prague 4 Bajzikova Martina Czech Republic Prague Hansikova Hana Czech Republic Pilsen Kuncova J Czech Republic Prague Mracek Tomas Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic Prague Pecina Petr Czech Republic Prague Fisar Zdenek Czech Republic Hradec Kralove Vernerova Andrea Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Aarhus Rattan Suresh IS Denmark Hvidovre Nehlin Jan O Denmark Copenhagen Rasmussen LJ Denmark Copenhagen Desler C Denmark Malov Hey-Mogensen M Denmark Copenhagen N Mortensen OH Egypt Cairo Ali Sameh S Egypt Cairo Abdel-Rahman Engy Ali Estonia Tallinn Truu Laura Estonia Tallinn Klepinina Lyudmila Estonia Tartu Grellier T Finland Jyvaeskylae Lehti Maarit Finland Turku Stier Antoine Finland Helsinki Pirinen E France Paris Petit Patrice X France Bron Rizwan Alam M France Bordeaux cedex Mourier A Germany Mainz Kalinovic S Germany Giessen Eckert Gunter P Germany Mainz Vujacic-Mirski Ksenija Germany Hamburg Singer Dominique Germany Duesseldorf Eckers A Germany Ulm Kolassa Iris Germany DΓΌsseldorf Prigione Alessandro Germany Duesseldorf Piel Sarah Germany Regensburg Renner-Sattler Kathrin Germany Ulm Schaefer PM Germany Freising Schoettl T Germany Leipzig Ost Mario Germany Freising Fromme Tobias Germany Freising Klingenspor Martin Germany Freising Keppner Gloria Germany Munich Jastroch M Germany Ulm Hartmann C Germany Freising-Weihenstephan Musiol Eva Germany Magdeburg Vieira Ligo Teixeira Camila Germany Ulm Calzia Enrico Germany Neuherberg Borchard Sabine Germany GieΓen Silaidos Carmina Germany Biberach an der RiΓ Nold V Greece Athens Trougakos Ioannis P Hungary Debrecen Verebne Tar K Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Ireland Dublin Porter Richard K Ireland Dublin Karavyraki Marilena Ireland Sligo Murphy J Israel Zrifin Sova Marina Israel Rishon Le Zion Hachmo Yafit Israel Jerusalem Saada Reisch Ann Italy Bolzano Pichler Irene Italy Bologna Porcelli AM Italy Bologna Gasparre G Italy Ancona Battino Maurizio Italy Bolzano/Bozen Volani Chiara Italy Bolzano Castelo Rueda Maria Paulina Italy Bologna Genova Maria Luisa Italy Verona Calabria Elisa Italy Rome Scatena Roberto Italy Padova Fernandez-Vizarra Erika Italy Bologna Paterlini S Italy Milano Bottoni P Japan Sapporo Shirakawa R Latvia Riga Kidere Dita Latvia Riga Makarova Elina Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Groningen Rots MG Netherlands Wageningen Keijer Jaap Netherlands Nijmegen Koopman Werner JH Norway Oslo Lund Jenny Norway Oslo Rustan AC Norway Oslo Nikolic N Norway Bergen Tronstad Karl Johan Norway Oslo Eide L Palestine Ramallah Stiban Johnny Poland Warsaw Sek Aleksandra Poland Warsaw Dymkowska Dorota Poland Poznan Michalak Slawomir Poland Lodz Watala Cezary Poland Lodz Labieniec-Watala Magdalena Poland Lodz Siewiera Karolina Poland Poznan Rybacka-Mossakowska Joanna Poland Krakow Kaczara P Poland PoznaΕ Ludziejewska A Poland Poznan Swiniuch Daria Poland Warsaw Wasilewski M Poland Warsaw Kampa Rafal Pawel Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Porto Beleza Jorge Portugal Porto Crisostomo Luis Portugal Porto Bernardino RL Portugal Coimbra Pereira Susana P Portugal Porto Carrageta D Portugal Porto Magalhaes Jose Portugal Porto Martins Ana Dias Portugal Coimbra Palmeira Carlos Portugal Coimbra Carvalho Eugenia Portugal Cantanhede Pinho Sonia A Portugal Porto Moreira Bruno P Portugal Porto Alves Marco G Portugal Cantanhede Bento Guida Jose Freitas Portugal CovilhΓ£ Dias Tania R Portugal Porto Oliveira Pedro Fontes Republic of Korea Daejeon Park SH Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Lighezan R Romania Timisoara Betiu AM Romania Timisoara Danila Maria-Daniela Romania Timisoara Lelcu Theia Romania Timisoara Avram Vlad F Romania Timisoara Muntean Danina M Russia Moscow Krylova T Russia Moscow Itkis YS Russia Moscow Tsygankova P Saudi Arabia Giza Kotb IA Serbia Belgrade Jeremic M Serbia Belgrade Misirlic-Dencic S Serbia Belgrade Ivanovic Tijana Serbia Belgrade Isakovic A Serbia Belgrade Vukojevic M Serbia Belgrad Markovic Ivanka Slovakia KoΕ‘ice Cizmarova Beata Slovakia Bratislava Sumbalova Zuzana Slovakia Bratislava Ukropec J Slovakia Bratislava Ukropcova B Slovakia Bratislava Maderova D Slovakia Bratislava Nemec Michal Slovenia Ljubljana Kopitar-Jerala Natasa Slovenia Ljubljana Pirkmajer Sergej Slovenia Ljubljana Mars T Spain Valencia Borras Consuelo Spain Valencia Victor Victor Manuel Spain Ourense Reboredo-Rodriguez Patricia Spain Sevilla Bustos Matilde Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Barcelona Dahdah Norma Ramon Spain Barcelona Arago Marc Spain Barcelona Gama Perez Pau Spain Valencia Fuertes Agudo Marina Spain Madrid Cadenas S Spain Barcelona Zorzano Antonio Spain Armilla, Granada Escames Germaine Spain A CoruΓ±a Blanco FJ Spain Valencia Casado Pinna Marta Spain Madrid Monsalve Maria Spain A CoruΓ±a Mayan MD Spain Granada Quiles JL Spain Madrid De los Rios C Spain Madrid Cano-Abad MF Spain Madrid Lopez MG Spain Barcelona Gonzalez Casacuberta I Spain Barcelona Juarez D Spain Barcelona Gil J Spain Salamanca Almeida Angeles Spain Lleida Nogueras L Spain Barcelona Tura Ceide O Spain Barcelona Hyrossova Petra Spain Lleida Gonzalo Hugo Spain Barcelona Smolders V Spain Sevilla Muntane Jordi Sweden SΓΆdertΓ€lje Alfaro-Moreno E Sweden LinkΓΆping Fontes-Oliveira CC Sweden Lund Chamkha Imen Sweden Lund Karlsson M Sweden Lund Abliva Sweden Lund Elmer Eskil Sweden Lund Westerlund E Sweden Lund Leffler M Sweden Lund Simon Serrano Sonia Sweden, SE Lund Aasander Frostner Eleonor Switzerland Geneva Martinou JC Switzerland Lausanne Zanou Nadege The Netherlands BT Amsterdam Wuest Rob CI Turkey Izmir Aydin HH Turkey Ankara Tuncay Erkan Turkey Istanbul Baykal AT Turkey Tekirdag Aral Cenk Turkey Ankara Engin Ayse Basak USA Orlando Coen Paul M USA Philadelphia Janowska Joanna USA Philadelphia Starr Jonathan USA Philadelphia Kilbaugh Todd USA Fayetteville Rao RR USA Iowa City Buettner Garry R USA Fayetteville Iyer Shilpa USA Baton Rouge Irving Brian A USA St Louis Welch R United Kingdom Brighton Moore Anthony L United Kingdom Stoke-on-Trent McStay Gavin P United Kingdom Leicester Moisoi Nicoleta United Kingdom Birmingham Joanisse S United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Birmingham Thakker A Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Projects in progress
- Β» MitoFit-Bloodcells
- Β» MiPMap