Difference between revisions of "Octanoylcarnitine"
From Bioblast
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::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
:::::* '''''Note 2024-03-26:''''' According to the information provided by the supplier (BioTrend) is the solubility in DMSO and H<sub>2</sub>O comparable, therefore we consider more rational approach to use H<sub>2</sub>O as a solvent due to higher melting point and possible toxic effects of DMSO, | :::::* '''''Note 2024-03-26:''''' According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H<sub>2</sub>O comparable, therefore we consider more rational approach to use H<sub>2</sub>O as a solvent for Octanoylcarnitine preparation due to higher melting point and possible toxic effects of DMSO, | ||
:::» '''O2k manual titrations into 2 mL O2k-chamber''' | :::» '''O2k manual titrations into 2 mL O2k-chamber''' |
Revision as of 18:45, 26 March 2024
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
MitoPedia topics: Substrate and metabolite
Application in HRR
- Oct: Octanoylcarnitine ((±)-Octanoylcarnitine chloride; C15H30NO4Cl), BioTrend (brand: APExBIO Technology): B6371-50 mg, store at -20 °C, CAS: 18822-86-1, M = 323.86 g·mol-1
- Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 g·mol-1.
- General tips: For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 °C.
- Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H2O comparable, therefore we consider more rational approach to use H2O as a solvent for Octanoylcarnitine preparation due to higher melting point and possible toxic effects of DMSO,
- » O2k manual titrations into 2 mL O2k-chamber
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 µL using a 10 µL Hamilton syringe
- Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
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MitoPedia:FAT4BRAIN