Permeabilized tissue or cells

high-resolution terminology - matching measurements at high-resolution

Permeabilized tissue or cells

Description

Permeabilized tissue (see permeabilized muscle fibres) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically, for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.

Permeabilized cells are, therefore, not any longer intact cells or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells.

Abbreviation: P_tic

Reference: MiPNet11.05; O2k-Publications: Permeabilized cells

MitoPedia methods: Respirometry