From Bioblast
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT
MitoPedia concepts:
MiP concept,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor,
Substrate and metabolite,
Uncoupler
Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.
SUIT protocols
- SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
- Attempts are in progress to develop a database of SUIT protocols