Ap5A

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Ap5A

Description

P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).

Abbreviation: Ap5A


Application in HRR

Ap5A: Ap5A (P1,P5-Di(adenosine-5β€²) pentaphosphate pentasodium salt; C20H29N10O22P5 Β· 5 Na), Sigma-Aldrich: D4022, store at -20 Β°C, CAS: 4097-04-5, M = 1026.28 gΒ·mol-1


Preparation of 100 mM stock solution (dissolved in H2O)
  1. Dissolve content of Sigma 2 mg glass vial in 19.49 Β΅L H2O
  2. Store at -20 Β°C.
Β» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 1 Β΅L using a 10 Β΅L Hamilton syringe.
  • Final concentration: 50 Β΅M.


References






MitoPedia topics: Inhibitor 

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