Banerjee 2018 Stem Cells Int

From Bioblast
Publications in the MiPMap
Banerjee A, Lindenmair A, Steinborn R, Dumitrescu SD, Hennerbichler S, Kozlov AV, Redl H, Wolbank S, Weidinger A (2018) Oxygen tension strongly influences metabolic parameters and the release of interleukine-6 of human amniotic mesenchymal stromal cells in vitro. Stem Cells Int 2018:9502451.

Β» Open Access

Banerjee A, Lindenmair A, Steinborn R, Dumitrescu SD, Hennerbichler S, Kozlov AV, Redl H, Wolbank S, Weidinger A (2018) Stem Cells Int

Abstract: The human amniotic membrane (hAM) has been used for tissue regeneration for over a century. In vivo (in utero), cells of the hAM are exposed to low oxygen tension (1–4 % oxygen), while the hAM is usually cultured in atmospheric, meaning high oxygen tension (20 % oxygen). We tested the influence of oxygen tensions on mitochondrial and inflammatory parameters of human amniotic mesenchymal stromal cells (hAMSCs). Freshly isolated hAMSCs were incubated for 4 days at 5 % and 20 % oxygen. We found 20 % oxygen to strongly increase mitochondrial oxidative phosphorylation, especially in placental amniotic cells. Oxygen tension did not impact levels of reactive oxygen species (ROS), however, placental amniotic cells showed lower levels of ROS, independent of oxygen tension. In contrast, the release of nitric oxide was independent of the amniotic region, but dependent on oxygen tension. Furthermore, IL-6 was significantly increased at 20 % oxygen. To conclude, short time cultivation at 20 % oxygen of freshly isolated hAMSCs induced significant changes in mitochondrial function and release of IL-6. Depending on the therapeutic purpose, cultivation conditions of the cells should be chosen carefully for providing the best possible quality of cell therapy. β€’ Keywords: Human amniotic mesenchymal cells, Oxygen, Mitochondria, Inflammation β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: IN Haldia Chakrabarti S, AT Vienna Kozlov AV

Labels: MiParea: Respiration, Comparative MiP;environmental MiP 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital  Preparation: Intact cells 

Coupling state: LEAK, ROUTINE, ET 

HRR: Oxygraph-2k 

Labels, 2018-09 

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