From Bioblast
- high-resolution terminology - matching measurements at high-resolution
Comparison of respirometric methods
Description
The comparison of respirometric methods provides the basis to evaluate different instrumental platforms and different mitochondrial preparations, as a guide to select the best approach and to critically evaluate published results.
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| MiPNet18.10 O2k-Specifications | 2024-03-26 |  | Human | Fibroblast |
| Zdrazilova 2022 PLOS ONE | 2022 | Zdrazilova L, Hansikova H, Gnaiger E (2022) Comparable respiratory activity in attached and suspended human fibroblasts. PLoS ONE 17:e0264496. https://doi.org/10.1371/journal.pone.0264496 | Human | Fibroblast |
| Gnaiger 2021 MitoFit BCA | 2021 | Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.08. https://doi.org/10.26124/mitofit:2021-0008 | Human | Fibroblast |
| Schmidt 2021 J Biol Chem | 2021 | Schmidt CA, Fisher-Wellman KH, Neufer PD (2021) From OCR and ECAR to energy: perspectives on the design and interpretation of bioenergetics studies. J Biol Chem 207:101140. https://doi.org/10.1016/j.jbc.2021.101140 | ||
| Perry 2013 Diabetes | 2013 | Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53. https://doi.org/10.2337/db12-1219 | Human | Skeletal muscle |
| Horan 2012 J Gerontol A Biol Sci Med Sci | 2012 | Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35. https://doi.org/10.1093/gerona/glr263 | ||
| Zhang 2012 Nat Protoc | 2012 | Zhang J, Nuebel E, Wisidagama DR, Setoguchi K, Hong JS, Van Horn CM, Imam SS, Vergnes L, Malone CS, Koehler CM, Teitell MA (2012) Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. https://doi.org/10.1038/nprot.2012.048 | Stem cells |
Discussion
MitoPedia methods: Respirometry
MitoPedia O2k and high-resolution respirometry:
O2k-Respirometry
