Graph options - DatLab

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O2k-Open Support

Graph options - DatLab



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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

DatLab 8: The โ€˜Graph optionsโ€™ window allows modifying graphs, scaling, plots and advanced features.

DatLab 7: Graph options, Add Graph/Delete bottom graph, Select plots, Scaling, Copy to clipboard


Reference: MitoPedia: DatLab

Under construction
Last update: 2024-05-27
Graphmenu.png

The Graph options window opens by default according to the selected graph and allows modifying scaling, plots and advanced features. The window can also be opened for any specific graph by left click on the corresponding action button on the right of the graph or by double clicking on y1, y2 or time axis. A different graph can be selected through the tabs on the left side of the window.

Abbreviation: F6

General options

Graphgenoptions.png

In the top right corner the following general options are displayed:

  • Add graph: Add a new graph below the last graph.
  • Delete graph: Delete the selected graph.
  • Save graph: Save the selected graph as an .svg file with the current displayed scaling, plots, events and marks. In this format, the graph elements can be ungrouped and individually edited.
  • Maximize graph: When checked the selected graph is maximized in the โ€˜Main windowโ€™ and is the only graph displayed. An active graph can also be maximized by double-clicking on it.

Scaling

The Scaling tab provides flexibility to vary the display of the plots. Viewing plots in differently scaled graphs, zooming the plots and time scales, and scrolling along the axes of the graph provide maximum information on the current experiment, but do not influence the format of stored data. Different ranges for the axes change the appearance of data dramatically and should be carefully adjusted.

Graphoptions-scaling.png
  • Range: defines the range of data displayed in the y1 axis (left), y2 axis (right) and x axis (Time).
  • Minimum: defines the minimum axis position for the display of data at a constant range.
  • Maximum: defines the maximum axis position for the display of data at a constant range.
The range value should be the first one defined. Once the range has been specified, any entry on the maximum or minimum value will adjust the other value accordingly.

The time unit displayed in the x axis can be changed in the drop-down menu (hh:mm; hh:mm:ss; seconds).

Synchronous time axis (on/off): When this option is โ€˜onโ€™ (green; as default) the time axis of all graphs is set at an identical range and offset (moves along together), which is particularly useful while panning. When this option is โ€˜offโ€™ (red) the time axis from all displayed graphs is independent. Synchtaxis.png

Custom label: When selected, allows customizing the name of the corresponding axis and unit name.

Autoscaley.png

Autoscale y1/ y2 axis (on/off): this option is โ€˜offโ€™ (light grey) by default, and, when selected (on; dark grey), the entire y1 or y2 axis range of the measurement is displayed in the graph.

Apply scalings: applies the scaling defined for the currently selected graph to another graph. The button from the currently select graph is therefore disabled.

Plots

The Plots tab allows managing and editing the plots that are displayed in a specific graph. All the standard layouts have plots with a default color.

Add plot: adds a new plot to the selected graph.

Graphoptions-plots.png

The following settings can be modified for each individual plot:

  • Channel defines the channel represented in the plot. In addition to the O2, Fluo, Amp, pX, Q, NADH, Photobiology channels, system channels can be selected. These include:
  • Barometric pressure: the continuously recorded absolute (local) barometric pressure [kPa].
  • Temperature: the continuously measured temperature of the copper block housing the two glass chambers [ยฐC].
  • Electronics humidity: signal from internal sensor recording the electronics humidity [%].
  • Electronics temperature: signal from internal temperature sensor recording the electronics temperature [ยฐC].
  • Temperature external channel: signal from external temperature sensor connected to the external temperature (temp ext.) plug in the back of the O2k.
  • Peltier power: for regulation of block temperature, continuously recorded [% of maximum power].
  • Room temperature: temperature from internal temperature sensor recording the environmental (room) temperature [ยฐC].
  • Data defines the type of data from the selected channel in the plot
  • Color defines the color of the plot
  • Left axis/ right axis defines the axis of the plot (left axis: y1; right axis: y2)
  • Chamber A/ Chamber B defines the chamber of the plot
  • Delete plot deletes the corresponding plot

Advanced

Graphoptions-advanced.png
  • Show marks: shows or hides the marks set in the selected graph, without deleting them.
  • Show events: shows or hides the events set in the selected graph, without deleting them.
  • Show instrument and notification events: shows or hides the instrumental and notification events automatically set by the software.
  • Allow zooming using the scroll-wheel in the graph: when selected, allows zooming in and out the time axis, when the mouse cursor is inside the graph, by rolling the scroll-wheel of the mouse (only enabled when automatic pan is off). The zooming is applied to all graphs displayed when the option โ€˜synchronous time axisโ€™ is selected. This feature should be used carefully and by experienced operators in order to avoid losing the reference of the recorded data.


Navigating the graph

The active graph/plot is selected by left click on the label of the plot on the right of the graph. The active graph is highlighted in white and indicated by the Oroboros logo in the background. Use arrow keys or the mouse to scroll (data on y-axes), pan (time on x-axis), or zoom (expansion or compression), independent of the โ€˜Graph optionsโ€™ window.

Mouse actions:

  • Dragging while left clicking on the y1, y2 or time axis of the active graph: allows moving the corresponding axis by keeping the range. The y1 and y2 are moved independently only in the active graph, while the time axis of all displayed graphs has the same offset if the 'synchronous time axis' option is selected.
  • Scroll-wheel on the y1, y2 or time axis of the active graph: allows zooming in and out the corresponding axis. The y1 and y2 are zoomed in and out independently only in the active graph, while the time axis of all displayed graphs has the same offset if the โ€˜synchronous time axis optionโ€™ is selected (See synchronous time axis)
  • Left clicking inside the graph and dragging left and right: moves the time axis left and right.
  • Double clicking inside the active graph: maximizes or minimizes the active graph.


Arrow keys:

  • ↑ or ↓ : Scrolls up and down the y axis of the active plot, with a shift of 50 %, keeping the range.
  • Ctrl+↑ : Zooms in the y axis of the active plot (the y axis range is halved).
  • Ctrl+↓ : Zooms out the y axis of the active plot (the y axis range is doubled).
  • → or ← : Scrolls left or right the time axis, with a shift of 50 %, keeping the range.
  • Ctrl+→ : Zooms in the time axis (expands the data, the original time range is halved). The upper limit of the time range is fixed.
  • Ctrl+← : Zooms out the time axis (compresses the data, the original time range is doubled). The reference time point is fixed on the right.



MitoPedia O2k and high-resolution respirometry: DatLab 

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