Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Hedges IBEC2012

From Bioblast
Hedges CP, Hickey AJ, McMahon CD, Smith HK (2012) A fiber-type-specific role for myostatin in regulating mitochondrial function. IBEC2012.

Link:

Hedges C, Hickey AJ, McMahon CD, Smith HK (2012)

Event: 15th International Biochemistry of Exercise Conference

Aim: This study aimed to compare mitochondrial oxygen consumption in C57BL/6J wild-type and myostatin-deficient mouse soleus and white gastrocnemius muscles.

Methods: Muscles were obtained from 4 month-old male mice. Mass-specific oxygen consumption (pmol.O2/mg/sec) was measured in permeabilised muscle, using high-resolution respirometry (Oroboros Oxygraph-2k). Maximal activities of citrate synthase and lactate dehydrogenase were determined by spectrophotometry.

Results: Myostatin-deficient soleus (n=6) consumed 20% more oxygen per mg than wild-type soleus (n=6) during oxidative phosphorylation. This was accompanied by greater citrate synthase and lactate dehydrogenase enzyme activity in myostatin-deficient soleus (29% and 80% respectively). Myostatin-deficient gastrocnemius (n=7) showed 26% lower oxygen consumption during uncoupled respiration, and 34% lower oxygen consumption when oxidising glycerol-3-phosphate compared to wild-type gastrocnemius (n=8). Citrate synthase activity was not significantly different and lactate dehydrogenase activity 26% greater in myostatin-deficient gastrocnemius compared to wild-type gastrocnemius.

Conclusion: These data suggest that myostatin-deficiency exerts fiber-type specific effects on skeletal muscle mitochondrial function.


β€’ O2k-Network Lab: NZ Auckland Hickey AJ, AU Melbourne Stepto NK


Labels: MiParea: Respiration, Genetic knockout;overexpression, Comparative MiP;environmental MiP 


Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: TCA cycle and matrix dehydrogenases  Regulation: Substrate  Coupling state: OXPHOS 

HRR: Oxygraph-2k, O2k-Spectrophotometer