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High signal at zero oxygen |
MitoPedia O2k and high-resolution respirometry:
Oroboros Open Support
Description
A high signal at zero oxygen may be observed during zero calibration (R0). First, check the quality of the dithionite solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current.
Reference: Zero calibration, OroboPOS-Connector
Leak current test
O2k-series J and XB
- Unplug the SmartPOS from the O2k-main unit.
- Remove the membrane from the SmartPOS and wash the cathode and anode with distilled water.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Connect the SmartPOS to the O2k-main unit. Set an event in DatLab called "SmartPOS".
- Record the signal for 10 min.
- The leak current of each individual component should be stable and below 0.001 Β΅A (DatLab 8). If the SmartPOS is giving a high leak current a sensor service frequently solves the problem. If you have any uncertainties, please forward the file to the technical support.
O2k-series D-I and XA
- Unplug the POS-Connector from the O2k-main unit and unscrew the OroboPOS from the POS-Connector. Screw the POS to the blue part of the perspex housing.
- Remove the membrane from the POS and wash the cathode and anode with distilled water.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
- Record the signal for 10 min.
- Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- Record the signal for 10 min.
- The leak current of each individual component should be stable and below 0.001 Β΅A (DatLab 8) or V (DatLab 7 and previous versions). Depending which component is giving a high leak current, a sensor service, followed by cleaning the electrical connections and drying the OroboPOS (without membrane) or the POS-Connector in an oven at up to 60 Β°C for 1-2 h frequently solves the problem. If you have any uncertainties, please forward the file to the technical support.
O2k-series A-C
- Plugging or unplugging of the POS-Connector while the main unit is switched on could damage the POS-Connector of O2k-series A-C. Therefore, the following should be started with the O2k switched off.
- Unplug the POS-Connector from the O2k-main unit and unscrew the OroboPOS from the POS-Connector. Screw the POS to the blue part of the perspex housing.
- Remove the membrane from the POS and wash the cathode and anode with distilled water.
- Plug the POS-Connector to the main unit.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- Record the signal for 10 min.
- The leak current of each individual component should be stable and below 0.001 V (DatLab 7 and previous versions). Depending which component is giving a high leak current, a sensor service, followed by cleaning the electrical connections and drying the OroboPOS (without membrane) or the POS-Connector in an oven at up to 60 Β°C for 1-2 h frequently solves the problem. If you have any uncertainties, please forward the file to the technical support.
References
Example
- First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the leak current.
- Figure 1. Leak current test. Upper panel: Leak current is in the range of Β±0.00099 V. Lower panel: Leak current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem of both, the OroboPOS-Connector, and the OroboPOS.
Keywords
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- Β» Oxygen signal
- Β» O2k signal line
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- General
- Β» Oxygen, dioxygen, O2
- Β» Oxygen calibration - DatLab
- Β» Oxygen solubility
- Β» Oxygen solubility factor
- Β» Oxygen pressure
- Β» Concentration
- Β» Activity
- Β» Pressure - Pascal
- Β» Barometric pressure
- Β» High-resolution respirometry
- Β» OroboPOS
- Β» Polarographic oxygen sensor
- Β» MitoFit Quality Control System
- Β» Sensitivity
- General
- Other keyword lists
MitoPedia O2k and high-resolution respirometry: Oroboros hardware, Oroboros Open Support, O2k-Respirometry