Horan 2012 J Gerontol A Biol Sci Med Sci

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Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35. https://doi.org/10.1093/gerona/glr263

Β» PMID: 22459622 Open Access

Horan MP, Pichaud N, Ballard JWO (2012) J Gerontol A Biol Sci Med Sci

Abstract: There is accumulating evidence that mitochondrial respiratory malfunction is associated with aging-associated complex diseases. However, progress in our understanding of these diseases has been hampered by the sensitivity and throughput of systems employed to quantify dysfunction and inherent limitations of the biological systems studied. In this review, we describe and contrast two methodologies that have been developed for measuring mitochondrial function to address the need for improved sensitivity and increased throughput. We then consider the utility of each methodology in studying three biological systems: isolated mitochondria, cultured cells, and cell fibers and tissues. Finally, we discuss the application of each methodology in the study of mitochondrial dysfunction in Alzheimer's disease, type 2 diabetes mellitus, and aging-associated autophagy impairment and mitochondrial malfunction. We conclude that the methodologies are complementary, and researchers may need to examine multiple biological systems to unravel complex diseases of aging. β€’ Keywords: Oroboros vs. Seahorse

β€’ O2k-Network Lab: AU Sydney Ballard JW

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Β» Comparison of respirometric methods

Cited by

  • Krako Jakovljevic N, Ebanks B, Katyal G, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. Bioenerg Commun 2021.2. https://doi.org/10.26124/bec:2021-0002

Labels: MiParea: Respiration, Instruments;methods, mt-Medicine  Pathology: Aging;senescence, Alzheimer's, Diabetes, Neurodegenerative 

HRR: Oxygraph-2k 

Comparison of respirometric methods, MitoFit2022QC 

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