Komlodi 2021 MitoFit Q

From Bioblast
Jump to navigation Jump to search


MitoFit Preprints         MitoFit Preprints        
Gnaiger 2019 MitoFit Preprints
       
Gnaiger MitoFit Preprints 2020.4
        MitoFit DOI Data Center         MitoPedia: Preprints


Komlodi 2021 MitoFit Q

Publications in the MiPMap
Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2.v4. doi:10.26124/mitofit:2021-0002.v4

»

MitoFit pdf

Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria

Komlodi Timea, Cardoso Luiza HD, Doerrier Carolina, Moore Anthony L, Rich Peter R, Gnaiger Erich (2021-09-15) MitoFit Prep

Abstract: Version 4 (v4) 2021-09-15 doi:10.26124/mitofit:2021-0002.v4

Version 3 (v3) 2021-09-01 doi:10.26124/mitofit:2021-0002.v3
Version 2 (v2) 2021-05-06 doi:10.26124/mitofit:2021-0002.v2
Version 1 (v1) 2021-02-18 doi:10.26124/mitofit:2021-0002 - »Link to all versions«

Redox states of the mitochondrial coenzyme Q pool, which reacts with the electron transfer system, reflect the balance between (1) reducing capacities of electron flow from fuel substrates converging at the Q-junction, (2) oxidative capacities downstream of Q to O2, and (3) the load on the OXPHOS system utilizing or dissipating the protonmotive force.

A three-electrode sensor (Rich 1988; Moore et al 1988) was implemented into the NextGen-O2k to monitor continuously the redox state of CoQ2 added as a Q-mimetic simultaneously with O2 consumption. The Q-Module was optimized for high signal-to-noise ratio, minimum drift, and minimum oxygen diffusion. CoQ2 equilibrates in the same manner as Q at Complexes CI, CII and CIII. The CoQ2 redox state is monitored amperometrically with the working electrode, which is poised at CoQ2 redox peak potentials determined by cyclic voltammetry. The voltammogram also provides quality control of the Q-sensor and reveals chemical interferences.

The CoQ2 redox state and O2 consumption were measured simultaneously in isolated mouse cardiac and brain mitochondria. CoQ2 ― and by implication mitochondrial Q ― was more oxidized when O2 flux was stimulated by coupling control: when energy demand increased from LEAK to OXPHOS and electron transfer capacities in the succinate pathway. In contrast, CoQ2 was more reduced when O2 flux was stimulated by pathway-control of electron input capacities, increasing from the NADH (N)- to succinate (S)-linked pathway which converge at the Q-junction, with CI-Q-CIII and CII-Q-CIII segments, respectively. N- and S- respiratory pathway capacities were not completely additive, compatible with partitioning of Q intermediary between the solid-state and liquid-state models of supercomplex organization. The direct proportionality of CoQ2 reduction and electron input capacities through the CI-Q-CIII and CII-Q-CIII segments suggests that CoQ2 is accurately mimicking mitochondrial Q-redox changes.

Keywords: coenzyme Q; CoQ, Q-junction, Q-redox state, electron transfer system; ETS, ETS-reactive Q-pool; Q, mitochondrial coenzyme Q; mtCoQ, supercomplexed Q; free Q-pool according to the fluid-state model; Qfree, cyclic voltammetry; CV, high-resolution respirometry; HRR, isolated mitochondria; imt, mouse heart mitochondria, mouse brain mitochondria, oxygen consumption, SUIT protocols, coupling control, pathway control, NS-pathway, additivity Bioblast editor: Komlodi T O2k-Network Lab: AT Innsbruck Oroboros

ORCID: ORCID.png Komlodi Timea, ORCID.png Cardoso Luiza HD, ORCID.png Doerrier Carolina, ORCID.png Rich Peter R, ORCID.png Gnaiger Erich

Data availability

Original files are available Open Access at Zenodo repository: 10.5281/zenodo.4478400

References

LinkReferenceViewYear
Aberg F, Appelkvist EL, Dallner G, Ernster L (1992) Distribution and redox state of ubiquinones in rat and human tissues. Arch Biochem Biophys 295:230-4.PMID:15861511992
Ausili A, Torrecillas A, Aranda F, de Godos A, Sánchez-Bautista S, Corbalán-García S, Gómez-Fernández JC (2008) Redox state of coenzyme Q10 determines its membrane localization. J Phys Chem B 112:12696-702.PMID:187957722008
Awad AM, Bradley MC, Fernandez-Del-Rio L, Nag A, Tsui HS, Clarke CF (2018) Coenzyme Q10 deficiencies: pathways in yeast and humans. Essays Biochem 62:361-76.PMID:29980630 Open Access2018
Balaban RS, Nemoto S, Finkel T (2005) Mitochondria, oxidants, and aging. Cell 120:483-95.PMID:15734681 Open Access2005
Benard G, Faustin B, Galinier A, Rocher C, Bellance N, Smolkova K, Casteilla L, Rossignol R, Letellier T (2008) Functional dynamic compartmentalization of respiratory chain intermediate substrates: implications for the control of energy production and mitochondrial diseases. Int J Biochem Cell Biol 40:1543-54.PMID:182074452008
Bentinger M, Brismar K, Dallner G (2007) The antioxidant role of coenzyme Q. Mitochondrion Suppl:S41-50.PMID:174828882007
Bianchi C, Fato R, Genova ML, Parenti Castelli F, Lenaz G (2003) Structural and functional organization of Complex I in the mitochondrial respiratory chain. Biofactors 18:3-9.PMID:146959152003
Bianchi C, Genova ML, Parenti Castelli G, Lenaz G (2004) The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysis. J Biol Chem 279:36562-9.PMID: 15205457 Open Access2004
Cohen ER, Cvitas T, Frey JG, Holmström B, Kuchitsu K, Marquardt R, Mills I, Pavese F, Quack M, Stohner J, Strauss HL, Takami M, Thor HL (2008) Quantities, Units and Symbols in Physical Chemistry. IUPAC Green Book 3rd Edition, 2nd Printing, IUPAC & RSC Publishing, Cambridge.Open Access2008
Cottingham IR, Moore AL (1983) Ubiquinone pool behaviour in plant mitochondria. Biochim Biophys Acta 724:191-200.[1]1983
Crane FL, Hatefi Y, Lester RL, Widmer C (1957) Isolation of a quinone from beef heart mitochondria. Biochim Biophys Acta 1000:362-3.PMID:26733861957
Crane FL, Sun IL, Clark MG, Grebing C, Low H (1985) Transplasma-membrane redox systems in growth and development. Biochim Biophys Acta 811:233-64.PMID:38935441985
Crane FL, Widmer C, Lester RL, Hatefi Y, Fechner W (1959) Studies on the electron transport system: XV. Coenzyme Q (Q275) and the succinoxidase activity of the electron transport particle. Biochim Biophys Acta 31:476-89.[2]1959
Crofts AR (2004) The cytochrome bc1 complex: function in the context of structure. Annu Rev Physiol 66:689-733.PMID:149774192004
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. doi: 10.1007/978-1-4939-7831-1_3PMID: 29850993 »O2k-brief
O2k-Protocols
2018
Dry IB, Moore AL, Day DA, Wiskich JT (1989) Regulation of alternative pathway activity in plant mitochondria: nonlinear relationship between electron flux and the redox poise of the quinone pool. Arch Biochem Biophys 273:148-57.PMID: 27573901989
Echtay KS, Winkler E, Klingenberg M (2000) Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature 408:609-13.PMID:111177512000
Enriquez JA, Lenaz G (2014) Coenzyme Q and the respiratory chain: coenzyme Q pool and mitochondrial supercomplexes. Mol Syndromol 5:119-40.PMID: 25126045 Open Access2014
Ernster L, Lee IY, Norling B, Persson B (1969) Studies with ubiquinone-depleted submitochondrial particles. Essentiality of ubiquinone for the interaction of succinate dehydrogenase, NADH dehydrogenase, and cytochrome b. Eur J Biochem 9:299-310.PMID: 4307591 Open Access1969
Estornell E, Fato R, Castelluccio C, Cavazzoni M, Parenti Castelli G, Lenaz G (1992) Saturation kinetics of coenzyme Q in NADH and succinate oxidation in beef heart mitochondria. FEBS Letters 311:107-9.1992
Fontaine E, Ichas F, Bernardi P (1998) A ubiquinone-binding site regulates the mitochondrial permeability transition pore. J Biol Chem 273:25734-40.PMID:97482421998
Gille L, Nohl H (2000) The existence of a lysosomal redox chain and the role of ubiquinone. Arch Biochem Biophys 375:347-54.PMID:107003912000
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97.PMID: 11718759
Bioblast pdf
2001
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.Bioblast pdf
O2k-Protocols contents
2008
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2: 112 pp. doi:10.26124/bec:2020-0002Bioblast pdfPublished online: 2020-Dec-30

2020
Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. doi:10.26124/mitofit:2021-0008
MitoFit pdf
Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts
2021-09-21
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Berlin, Heidelberg:431-42. https://doi.org/10.1007/978-3-662-04162-8_45Bioblast pdf - Springer link
O2k-Protocols contents
2000
Graham D (2018) Standard operating procedures for cyclic voltammetry. ISBN 978-1-387-51430-4.http://sop4cv.com/2018
Gulaboski R, Markovski V, Jihe Z (2016) Redox chemistry of coenzyme Q—a short overview of the voltammetric features. J Solid State Electrochem 20:3229–3238.[3]2016
Gutman M (1985) Kinetic analysis of electron flux through the mitochondrial system. Coenzyme Q, Chichester, UK: Wiley 10:215-34.[4]1985
Hackenbrock CR, Chazotte B, Gupte SS (1986) The random collision model and a critical assessment of diffusion and collision in mitochondrial electron transport. J Bioenerg Biomembr 18:331–68.PMID:30217141986
Hatefi Y, Lester RL, Crane FL, Widmer C (1959) Studies on the electron transport system. XVI. Enzymic oxidoreduction reactions of coenzyme Q. Biochim Biophys Acta 31:490-501.PMID:136286781959
Havaux M (2020) Plastoquinone in and beyond photosynthesis. Trends Plant Sci 25:1252-65.PMID: 327137762020
Hernández-Camacho JD, Bernier M, López-Lluch G, Navas P (2018) Coenzyme Q(10) Supplementation in aging and disease. Front Physiol 9:44.PMID:294598302018
Hunte C, Palsdottir H, Trumpower BL (2003) Protonmotive pathways and mechanisms in the cytochrome bc1 complex. FEBS Letters 545:39-46.PMID:127884902003
James AM, Cochemé HM, Smith RAJ, Murphy MP (2005) Interactions of mitochondria-targeted and untargeted ubiquinones with the mitochondrial respiratory chain and reactive oxygen species. Implications for the use of exogenous ubiquinones as therapies and experimental tools. J Biol Chem 280:21295-312.PMID:157883912005
Jørgensen BM, Rasmussen HN, Rasmussen UF (1985) Ubiquinone reduction pattern in pigeon heart mitochondria. Identification of three distinct ubiquinone pools. Biochem J 229:621-9.PMID:4052014 Open Access1985
Kalén A, Appelkvist EL, Dallner G (1987) Biosynthesis of ubiquinone in rat liver. Acta Chem Scand B 41:70-2.PMID:35775541987
Kröger A, Klingenberg M (1966) On the role of ubiquinone in mitochondria. II. Redox reactions of ubiquinone under the control of oxidative phosphorylation. Biochem Z 344:317-36.PMID:42958561966
Kröger A, Klingenberg M (1973) Further evidence for the pool function of ubiquinone as derived from the inhibition of the electron transport by antimycin. Eur J Biochem 39:313-23.PMID:4359626 Open Access1973
Kröger A, Klingenberg M (1973) The kinetics of the redox reactions of ubiquinone related to the electron-transport activity in the respiratory chain. Eur J Biochem 34:358-68.PMID:4351161 Open Access1973
Laboratory Protocol: isolation of rat brain mitochondria.Bioblast pdf »Versions
O2k-Protocols
2016-06-18
Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8PMID: 28588260 Sci Rep Open Access2017
Lenaz G, Genova ML (2009) Structural and functional organization of the mitochondrial respiratory chain: A dynamic super-assembly. Int J Biochem Cell Biol 41:1750-72.PMID:19711505 Open Access2009
Lopez-Lluch G, Rodriguez-Aguilera JC, Santos-Ocana C, Navas P (2010) Is coenzyme Q a key factor in aging? Mech Ageing Dev 131:225-35.PMID:201937052010
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-75.PMID:149077131951
Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191:144-8.PMID: 137713491961
Mitchell P (1975) The protonmotive Q cycle: A general formulation. FEBS Lett 59:137-9.Open Access1975
Moore AL, Dry IB, Wiskich JT (1991) Regulation of electron transport in plant mitochondria under state 4 conditions. Plant Physiol 95:34-40.PMID: 16667977 Open Access1991
Moore AL, Dry IB, Wiskich TJ (1988) Measurement of the redox state of the ubiquinone pool in plant mitochondria. FEBS Lett 235:76-80.Open Access1988
Morré DJ, Morré DM (1989) Preparation of mammalian plasma membranes by aqueous two-phase partition. Biotechniques 7:946-58.PMID:24836651989
Morré DJ, Morré DM (2011) Non-mitochondrial coenzyme Q. Biofactors 37:355-60.PMID:216746412011
Noh YH, Kim K-Y, Shim MS, Choi S-H, Choi S, Ellisman MH, Weinreb RN, Perkins GA, Ju W-K (2013) Inhibition of oxidative stress by coenzyme Q10 increases mitochondrial mass and improves bioenergetic function in optic nerve head astrocytes. Cell Death Dis 4:e820.PMID:24091663 Open Access2013
Nyquist SE, Barr R, Morré DJ (1970) Ubiquinone from rat liver Golgi apparatus fractions. Biochim Biophys Acta 208:532-4.PMID:55065811970
Osakai T, Yamamoto T, Ueki M (2019) Directional electron transfer from ubiquinone-10 to cytochrome c at a biomimetic self-assembled monolayer modified electrode. Electrochemistry 87:59-64.Open Access2019
Petrova EV, Korotkova EI, Kratochvil B, Voronova OA, Dorozhko EV, Bulycheva EV (2014) Investigation of coenzyme Q10 by voltammetry. Proc Chem 10:173-8. https://doi.org/10.1016/j.proche.2014.10.030.Open Access2014
Rauchová H, Fato R, Drahota Z, Lenaz G (1997) Steady-state kinetics of reduction of coenzyme Q analogs by glycerol-3-phosphate dehydrogenase in brown adipose tissue mitochondria. Arch Biochem Biophys 344:235-41.PMID:9244403 Open Access1997
Reed JS, Ragan CI (1987) The effect of rate limitation by cytochrome c on the redox state of the ubiquinone pool in reconstituted NADH: cytochrome c reductase. Biochem J 247:657-62.PMC:11484621987
Rich PR (1982) Electron and proton transfers in chemical and biological quinone systems. Faraday Discuss Chem Soc 74:349-64.PMID:71834551982
Rich PR (1984) Electron and proton transfers through quinones and cytochrome bc complexes. Biochim Biophys Acta 768:53-79.PMID: 63228441984
Rich PR (1988) Patent of Q-electrode. Glynn Res. Ph. European Patent no.85900699.1.1988
Rich PR (2004) The quinone chemistry of bc complexes. Biochim Biophys Acta 1658:165-71.PMID:15282188 Open Access2004
Rodríguez-Hernández A, Cordero MD, Salviati L, Artuch R, Pineda M, Briones P, Izquierdo LG, Cotán D, Navas P, Sánchez-Alcázar JA (2009) Coenzyme Q deficiency triggers mitochondria degradation by mitophagy. Autophagy 5:19-32.PMID: 19115482 Open Access2009
Sastry Seshadri P, Jayaraman J, Ramasarma T (1961) Distribution of coenzyme Q in rat liver cell fractions. Nature 189:577.Open Access1961
Selected media and chemicals for respirometry with mitochondrial preparations.Bioblast pdf  »Versions
O2k-Protocols
2016-08-30
Stoner CD (1984) Steady-state kinetics of the overall oxidative phosphorylation reaction in heart mitochondria. Determination of the coupling relationships between the respiratory reactions and miscellaneous observations concerning rate-limiting steps. J Bioenerg Biomembr 16:115-41.PMID:61002961984
Takada M, Ikenoya S, Yuzuriha T, Katayama K (1984) Simultaneous determination of reduced and oxidized ubiquinones. Methods Enzymol 105:147-55.1984
Tang PH, Miles MV (2012) Measurement of oxidized and reduced coenzyme Q in biological fluids, cells, and tissues: an HPLC-EC method. Methods Mol Biol 837:149-68.PMID:222155462012
Trumpower BL (1990) The protonmotive Q cycle. Energy transduction by coupling of proton translocation to electron transfer by the cytochrome bc1 complex. J Biol Chem 265:11409-12.PMID:21640011990
Trumpower BL, Gennis RB (1994) Energy transduction by cytochrome complexes in mitochondrial and bacterial respiration: the enzymology of coupling electron transfer reactions to transmembrane proton translocation. . Annu Rev Biochem 63:675-716.PMID:79792521994
Turunen M, Olsson J, Dallner G (2004) Metabolism and function of coenzyme Q. Biochim Biophys Acta 1660:171-99.PMID:14757233 Open Access2004
Urban PF, Klingenberg M (1969) On the redox potentials of ubiquinone and cytochrome b in the respiratory chain. Eur J Biochem 9:519-25.Open Access1969
Van den Bergen CW, Wagner AM, Krab K, Moore AL (1994) The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interplay between quinol-oxidizing and quinone-reducing pathways. Eur J Biochem 226:1071-8.PMID: 7813462 Open Access1994
Watts JL, Ristow M (2017) Lipid and Carbohydrate Metabolism in Caenorhabditis elegans. Genetics 207:413-46.PMID:28978773 Open Access2017
Wolf DE, Hoffman CH, Trenner NR, Arison BH, Shunk CH, Linn BO, McPherson JF, Folkers K (1958) Coenzyme Q. I. Structure studies on the coenzyme Q group. J Am Chem Soc 80:4752.Open Access1958
Wu F, Yang F, Vinnakota KC, Beard DA (2007) Computer modeling of mitochondrial tricarboxylic acid cycle, oxidative phosphorylation, metabolite transport, and electrophysiology. J Biol Chem 282:24525-37.Open Access2007
Zannoni D, Moore AL (1990) Measurement of the redox state of the ubiquinone pool in Rhodobacter capsulatus membrane fragments. FEBS Lett 271:123-7.PMID: 2171997 Open Access1990
O2k-Manual
Mitochondrial Respiration Medium - MiR05-Kit.
Bioblast pdf » Versions2019-09-02
O2k-Protocols
Laboratory protocol: isolation of mouse heart mitochondria.
Bioblast pdf »Versions2021-08-09
O2k-Protocols
NextGen-O2k: Q-Module manual
PDF.jpg » Versions2021-06-24

Keywords


Questions.jpg


Click to expand or collaps
Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>


Cited by

  • Komlodi et al (2021) Hydrogen peroxide production, mitochondrial membrane potential and the redox state of the coenzyme Q measured at tissue normoxia and experimental hyperoxia in heart mitochondria. MitoFit Preprints 2021 (in prep)

Support

Template NextGen-O2k.jpg
Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.


Labels: MiParea: Respiration, Instruments;methods 


Organism: Mouse  Tissue;cell: Heart, Nervous system  Preparation: Isolated mitochondria 

Regulation: Redox state, Q-junction effect  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS  HRR: Oxygraph-2k, NextGen-O2k 

SUIT-006 Q mt D071, SUIT-006 Q ce-pce D073, SUIT-031 Q mt D072, SUIT-031 Q ce-pce D074, MitoFit 2021 Photosynthesis, MitoFit 2021 Tissue normoxia