O2k-Demo experiments

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O2k-Open Support

O2k-Demo experiments


O2k-Procedures
SUIT-Protocol

Oroboros O2k demo

ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Gnaiger 2020 BEC MitoPathwaysOpen Access pdf published online 2020-12-30

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002Permeabilized cells
Permeabilized tissue
Homogenate
Isolated mitochondria
Intact cells
Heart
Skeletal muscle
Fibroblast
Human
Mouse
2020
MiPNet12.01 Suppl T-issueBioblast pdf Β»Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
Permeabilized tissueSkeletal muscleMouse2018-11-18
MiPNet21.14 Reference sample HRRBioblast pdf Β»Versions
O2k-Protocols
Development of a reference sample for HRR.
Intact cells
Permeabilized cells
HEKHuman2016-10-19
MiPNet08.09 CellRespirationBioblast pdf  Β»Versions
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
Intact cellsBlood cells
Lymphocyte
Human2016-08-11
MiPNet17.03 Shredder vs FibersBioblast pdf Β»Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
HomogenateHeart
Liver
Fishes2015-06-18
MiPNet12.11 MitoRespirationBioblast pdf Β»Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Isolated mitochondriaArtemia
Crustaceans
2014-02-20
Pesta 2012 Methods Mol BiolPMID: 22057559
Bioblast pdf
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3Permeabilized cells
Permeabilized tissue
Intact cells
Skeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Human2012
MiPNet08.12 IOC22Bioblast pdf
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
Isolated mitochondriaLiverRat2011-12-11
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.Permeabilized cells
Permeabilized tissue
Intact cells
Blood cellsMouse2008
MiPNet04.05 Titration-InjectionBioblast pdf
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
Intact cellsEndothelial;epithelial;mesothelial cell
HUVEC
Human1999-01
TIP2k-Module

O2k-Demo TIP2k

Β» O2k-Catalogue: TIP2k
Β» O2k-Publications: TIP2k
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Pesta 2012 Methods Mol BiolPMID: 22057559
Bioblast pdf
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3Permeabilized cells
Permeabilized tissue
Intact cells
Skeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Human2012
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.Permeabilized cells
Permeabilized tissue
Intact cells
Blood cellsMouse2008
MiPNet04.05 Titration-InjectionBioblast pdf
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
Intact cellsEndothelial;epithelial;mesothelial cell
HUVEC
Human1999-01

TIP2k Manual

ChapterViewSectionLast update
MiPNet12.10 TIP2k-manualBioblast pdf Β»Versions
O2k-Manual
Titration-Injection microPump TIP2k manual.
2021-07-08



O2k-FluoRespirometer
O2k-TPP-ISE.jpg

O2k-Demo O2k-MultiSensor

Β» O2k-Catalogue: O2k-MultiSensor
Β» O2k-Publications: O2k-Fluorometry
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Komlodi 2021 BEC AmR-O2Bioenerg Commun 2021.4. Open Access pdf published online 2021-12-21

KomlΓ³di T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004Intact cellsOther cell linesSaccharomyces cerevisiae2021
MiPNet24.11 mtMP calculationPDF.jpg Β» Versions
O2k-Protocols
Mitochondrial membrane potential calculation
2019-08-05
MiPNet20.14 AmplexRed H2O2-productionBioblast pdf Β»Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
Isolated mitochondriaHeartMouse2019-06-24
MiPNet20.13 Safranin mt-membranepotentialBioblast pdf Β»Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
HomogenateNervous systemMouse2019-06-24
MiPNet18.05 Amplex-Mouse-heartBioblast pdf Β»Versions
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
HomogenateHeartMouse2019-06-24
Krumschnabel 2015 Methods Mol BiolPMID: 25631019Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.Isolated mitochondriaNervous systemMouse2015
Makrecka-Kuka 2015 BiomoleculesBiomolecules 5:1319-38. PMID: 26131977 Open Access
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319Permeabilized cells
Homogenate
Isolated mitochondria
Intact cells
Heart
Nervous system
HEK
Human
Mouse
2015
Krumschnabel 2014 Methods EnzymolPMID: 24862266 Β»O2k-brief
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1HomogenateNervous systemMouse2014
MiPNet08.12 IOC22Bioblast pdf
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
Isolated mitochondriaLiverRat2011-12-11

O2k-MultiSensor Manual

ChapterViewSectionLast update
MiPNet15.03 O2k-MultiSensor-ISEBioblast pdf Β»Versions
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2021-08-02
MiPNet15.05 NO-manualBioblast pdf  Β»Versions
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-ModuleBioblast pdf Β»Versions
O2k-Manual
O2k-Fluo LED2-Module.
2021-06-22
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08
MiPNet24.12 NextGen-O2k: Q-ModulePDF.jpg Β» Versions
O2k-Manual
NextGen-O2k: Q-Module manual
2021-10-29
MiPNet26.11 NextGen-O2k: PB-ModulePDF.jpg Β» Versions
O2k-Manual
NextGen-O2k: PB-Module manual
2021-10-29
MiPNet26.12 NextGen-O2k: NADH-ModulePDF.jpg Β» Versions
O2k-Manual
NextGen-O2k: NADH-Module manual
2022-05-17
MiPNet28.09 O2k-Fluo Smart-Module manualPDF.jpg Β» Versions
O2k-Manual
O2k-Fluo Smart-Module manual
2023-09-25


O2k-Guides: Β»O2k-Catalogue :: Β»O2k-Manual :: Β»O2k-Publications

O2k-Procedures
O2k-Procedures
Β» O2k-Procedures: MitoPathways
Pesta 2012 Methods Mol Biol Fig7A.jpg
Β» O2k-Demo experiments
Pesta 2012 Methods Mol Biol Fig7A.jpg
Β» O2k-Analysis
O2k-Procedures
Β» O2k-Chemicals and media
PBI-Shredder O2k-Set
Β» O2k-mitochondrial preparations
O2k-Protocols
Β» O2k-Procedures: Mitochondrial and marker-enzymes
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