Oroboros Demo experiments

Oroboros Demo

Protocol	View	Topic	Preparation	Tissue and cell	Mammal and model	Last update
Gnaiger 2020 BEC MitoPathways	published online 2020-12-30	Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002	Permeabilized cells Permeabilized tissue Homogenate Isolated mitochondria Intact cells	Heart Skeletal muscle Fibroblast	Human Mouse	2020
MiPNet17.03 Shredder vs Fibers	»Versions	Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.	Homogenate	Heart Liver	Fishes	2015-06-18
MiPNet12.11 MitoRespiration	»Versions	Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.	Isolated mitochondria		Artemia Crustaceans	2014-02-20
Pesta 2012 Methods Mol Biol	PMID: 22057559	Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3	Permeabilized cells Permeabilized tissue Intact cells	Skeletal muscle Other cell lines HEK Fibroblast HUVEC	Human	2012
MiPNet08.12 IOC22		NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.	Isolated mitochondria	Liver	Rat	2011-12-11
Gnaiger 2008 POS		Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.	Permeabilized cells Permeabilized tissue Intact cells	Blood cells	Mouse	2008

Demo TIP2k

» TIP2k-Module

» O2k-Publications: TIP2k

Protocol	View	Topic	Preparation	Tissue and cell	Mammal and model	Last update
Donnelly 2024 Redox Biol	PMID: 38401291 Open Access	Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion A-C, Matera A, Tavernari D, Campiche O, Paolicelli RC, Zanou N, Kayser B, Gnaiger E, Place N (2024) Functional hypoxia reduces mitochondrial calcium uptake. Redox Biol 71:103037. https://doi.org/10.1016/j.redox.2024.103037	Intact organism Isolated mitochondria Intact cells	Heart Skeletal muscle	Human Mouse	2024
Pesta 2012 Methods Mol Biol	PMID: 22057559	Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3	Permeabilized cells Permeabilized tissue Intact cells	Skeletal muscle Other cell lines HEK Fibroblast HUVEC	Human	2012
Gnaiger 2008 POS		Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.	Permeabilized cells Permeabilized tissue Intact cells	Blood cells	Mouse	2008
MiPNet04.05 Titration-Injection		From step titration to ramp injection: Uncoupling by FCCP with TIP.	Intact cells	Endothelial;epithelial;mesothelial cell HUVEC	Human	1999-01

Demo MultiSensor

» O2k-Publications: O2k-Fluorometry

Protocol	View	Topic	Preparation	Tissue and cell	Mammal and model	Last update
Komlodi 2021 BEC AmR-O2	Bioenerg Commun 2021.4. published online 2021-12-21	Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004	Intact cells	Other cell lines	Saccharomyces cerevisiae	2021
MiPNet20.14 AmplexRed H2O2-production	»Versions	O2k-FluoRespirometry: HRR and simultaneous determination of H₂O₂ production with Amplex UltraRed.	Isolated mitochondria	Heart	Mouse	2019-06-24
MiPNet20.13 Safranin mt-membranepotential	»Versions	O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.	Homogenate	Nervous system	Mouse	2019-06-24
MiPNet18.05 Amplex-Mouse-heart	»Versions	O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate.	Homogenate	Heart	Mouse	2019-06-24
Krumschnabel 2015 Methods Mol Biol	PMID: 25631019	Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. https://doi.org/10.1007/978-1-4939-2257-4_22	Isolated mitochondria	Nervous system	Mouse	2015
Makrecka-Kuka 2015 Biomolecules	PMID: 26131977 Open Access	Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. https://doi.org/10.3390/biom5031319	Permeabilized cells Homogenate Isolated mitochondria Intact cells	Heart Nervous system HEK	Human Mouse	2015
Krumschnabel 2014 Methods Enzymol	PMID: 24862266 »	Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1	Homogenate	Nervous system	Mouse	2014
MiPNet08.12 IOC22		NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.	Isolated mitochondria	Liver	Rat	2011-12-11

	Oroboros Procedures
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