From Bioblast

Bioblast 2022


Tyrolean Government grant
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  • The project O2k-Innovation (officially NextGen-O2k project) was supported by the "Technologiefรถrderungsprogramm - Tiroler Innovationsfรถrderung" of the Tyrolean Government.
  • Project duration: 2015-02-01 to 2017-01-31
  • Partners in the Oroboros Ecosystem: WGT Elektronik, Software Security Networks
  • Application: 2015-01-30; Granted: 2015-03-24 (total budget โ‚ฌ 350.000)
  • Funding: โ‚ฌ 140,000 plus a bonus of โ‚ฌ 5,000 for the implementation of gender sensitive actions

O2k-Innovation publication

  • Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - ยปBioblast linkยซ

The fourth dimension of respiratory control

  1. On the "line": one dimension is insufficient.
  2. Coupling and pathway control are two necessarily connected dimensions defining the โ€œareaโ€ of respiratory control.
  3. Adding kinetics (including substrate kinetics of O2, CHO, adenylates, Pi, and flux-force kinetics) is the third dimension bringing โ€œvolumeโ€ into the picture of classical mitochondrial respiratory control.
  4. The O2k-Innovation on top of O2k-MultiSensor modules and the O2k-FluoRespirometer represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca2+, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O2 and O2-flux in the same chamber.

Development of the O2k-Innovation prototype: a look inside

Different stages of prototype development: The O2k-Innovation is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.
The NextGen-O2k builds upon and extends the concept of the O2k-Fluorometer elaborated within the K-Regio Project MitoCom O2k-Fluorometer (2011-2014). To broaden the specifications of the O2k-FluoRespirometer, the NextGen-O2k combines high-resolution respirometry with a Q-Module and NADH-Module for RedoxBiology and the PB-Module to study PhotoBiology (including photosynthesis). It is our ultimate goal to further extend the NextGen-O2k with O2k-Spectrofluorometry (including ratiometric measurements), and O2k-Spectrophotometry (allowing for simultaneous measurement of redox states of the cytochromes aa3, b, and c).
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