Oroboros Workshops

Oroboros Workshops

Oroboros Workshops are organized as international oxygraph courses (IOC) on high-resolution respirometry since 1991. These range from four full-day training courses to conference lunch presentations of the Oroboros.

Next Oroboros Workshops

Next Oroboros Workshops

	Date	Location - Country - IOC
OroW2025-06 IOC Schroecken AT	2025-06-23	Schroecken AT, 2025 Jun 23 - 28. Oroboros Workshop on high-resolution respirometry (HRR), IOC.
OroW2025-09 IOC Schroecken AT	2025-09-29	Schroecken AT, 2025 Sep 29 - Oct 04. Oroboros Workshop on high-resolution respirometry (HRR), IOC.

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General contents of Oroboros Workshops

Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.
High-resolution respirometry.
Optimum system design - the Oroboros.
DatLab: the specialized software for high-resolution respirometry and high-resolution calibrations with real-time recording.
Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.

Oroboros and TIP2k: Real-time instrumental performance

Instrumental background oxygen flux: measurement and correction as a function of p_O2.
High-resolution of respiratory flux at various steady-states, SUIT protocols.
Titration-Injection microPump (TIP2k): automatic titrations.
Oroboros and TIP2k: instrumental maintenance.
Conceptual and methodological advantages of measurement at physiological low levels of oxygen.

Polarographic oxygen sensor (POS) and Oroboros service

Cleaning of anode and cathode.
Electrolyte and membrane application.

Fluo Smart-Module

Formats

» Virtual Oroboros Installation and startup support session

» Oroboros Workshops in Schroecken (Austria)

» Oroboros Coaching Days Innsbruck

» Virtual Oroboros Workshops

Oroboros Workshops have become world-famous. They are offered on a regular basis in Austria and in other countries in cooperation with O2k-Network Labs.

Oroboros Workshop: HRR and the Oroboros FluoRespirometer

The Oroboros Workshop on high-resolution respirometry provides a detailed introduction to the Oroboros, overview presentations on [high-resolution respirometry]] (HRR), and applications in mitochondrial physiology.

The Oroboros Workshop program is complemented by lectures on mitochondrial physiology (general topics; Oroboros Procedures). Short presentations of 'Oroboros perspectives' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.

Oroboros Workshop: Advanced Oroboros FluoRespirometry

The Advanced Oroboros FluoRespirometry Workshop is focused on fluorometry combined with respirometry. High-resolution respirometry (HRR) is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin), ATP production (Magnesium green), and Ca²⁺ (Calcium green). Further Oroboros MultiSensor applications are covered in these specific Oroboros Workshops (e.g. IOC95).

Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Oroboros perspectives'.

Information on Oroboros FluoRespirometry, with resolution of hydrogenperoxide in the nanomolar range: Oroboros FluoRespirometer.

Oroboros Workshop: TPP/ ISE

The Oroboros Workshop TPP/ISE is customized for participants interested in MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of mitochondrial membrane potential (TPP+, TPMP+) and Ca²⁺. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP⁺ to mt-membrane potential) constitutes a major topic.

According to the interest of participants, other Oroboros MultiSensor applications can be discussed and demonstrated:

Measurement of Ca²⁺, calibration and calculation of free Ca²⁺ in buffered solutions (TPP ISE-Module)
Acidification rate (pH)
Oroboros FluoRespirometer
Nitric Oxide (NO) Module

Oroboros Workshops in O2k-Network Labs

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Abstracts

Abstracts are welcome for short presentations, providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.

Abstracts should be submitted to [email protected]

Instructions for IOC-Abstracts

Abstracts are welcome for short presentations (Oroboros perspectives), providing participants an opportunity to present and discuss their work, including projects in progress or in the planning state, methodological details and conceptual aspects. Presentation duration: 10 min presentation + 5 min questions

Length of text: Max. 500 words including up to three references, all authors with full titles, their affiliations, and a figure legend. The abstract does not allow excess length of text. Additional information (further references etc.) may be added into the free text which will not be included should there be a printed version.
You may edit your abstract on the website any time. Please send us your abstract two weeks prior to the Oroboros Workshop. Should you have last-minute ideas or changes on your abstract please get in touch with the tutors of the Oroboros Workshop.
Kindly submit your abstract by emailing it to us in a wordfile. We will then add your abstract to the Bioblast wiki. Please let us know if you do not wish your abstract to be open access.
Figures: A figure is welcome. Please send us a figure (jpg or png).

Oroboros travel grant

Oroboros Instruments offers travel grants for the support of scientists attending the Oroboros Workshop on high-resolution respirometry.

Eligibility and Application

Depending on the Oroboros package you purchased, you may be eligible for receiving an Oroboros Travel Grant. Please contact Oroboros Instruments for further information.

General Information