| Oliveira MF, Aveiro Y, Couto-Lima CA (2023) A navigation chart to avoid turbulence in Drosophila mitochondrial research. Bioenerg Commun 2023.4. https://doi.org/10.26124/bec:2023-0004 |
» Bioenerg Commun 2023.04. 
published online 2023-11-06
Oliveira Marcus F, Aveiro Yan, Couto-Lima Carlos A (2023) Bioenerg Commun
Abstract: 
https://doi.org/10.26124/bec:2023-0004
Drosophila fruit flies have been used as a valuable, cheap, and powerful model organism to understand fundamental biological processes for many years. However, standardized methodologies specifically designed to assess mitochondrial physiology in this model are not available. Rodríguez and colleagues provided a detailed analysis of publicly available protocols to assess mitochondrial physiology in Drosophila melanogaster and performed experiments in flight muscles to address three technical parameters to define the optimal conditions for respirometry. The authors show that oxygen diffusion is not limited to sustaining respiratory capacity in either isolated mitochondria or chemically permeabilized fibers. In addition, chemical permeabilization was revealed as the best approach to assess mitochondrial physiology in fruit flies. Finally, the authors demonstrate that magnesium green is the only fluorescent probe that caused no effects on respiratory rates. Methodological standardization to study Drosophila mitochondrial physiology, as presented by Rodríguez and colleagues, represents a critical step towards more reproducible and comparative metabolic research in this important model organism. • Keywords: Drosophila; method; mitochondria; standard; permeabilization; oxygen diffusion; probe • Bioblast editor: Tindle-Solomon L • O2k-Network Lab: BR Rio de Janeiro Oliveira MF
ORCID: 
Oliveira Marcus F, Aveiro Yan, Couto-Lima Carlos A
MitoFit Preprint
Labels: MiParea: Respiration
Organism: Drosophila
Preparation: Permeabilized tissue, Homogenate
BEC
