Oliveira 2023 BEC

From Bioblast


Bioenergetics Communications        
Tributes to pioneers in bioenergetics
       
Gnaiger 2020 BEC MitoPathways
       
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
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Bioenergetics Communications
Publications in the MiPMap
Oliveira MF, Aveiro Y, Couto-Lima CA (2023) A navigation chart to avoid turbulence in Drosophila mitochondrial research. Bioenerg Commun 2023.4. https://doi.org/10.26124/bec:2023-0004

Β» Bioenerg Commun 2023.04. Open Access pdf
published online 2023-11-06

Oliveira Marcus F, Aveiro Yan, Couto-Lima Carlos A (2023) Bioenerg Commun

Abstract: BEC.png https://doi.org/10.26124/bec:2023-0004

Drosophila fruit flies have been used as a valuable, cheap, and powerful model organism to understand fundamental biological processes for many years. However, standardized methodologies specifically designed to assess mitochondrial physiology in this model are not available. RodrΓ­guez and colleagues provided a detailed analysis of publicly available protocols to assess mitochondrial physiology in Drosophila melanogaster and performed experiments in flight muscles to address three technical parameters to define the optimal conditions for respirometry. The authors show that oxygen diffusion is not limited to sustaining respiratory capacity in either isolated mitochondria or chemically permeabilized fibers. In addition, chemical permeabilization was revealed as the best approach to assess mitochondrial physiology in fruit flies. Finally, the authors demonstrate that magnesium green is the only fluorescent probe that caused no effects on respiratory rates. Methodological standardization to study Drosophila mitochondrial physiology, as presented by RodrΓ­guez and colleagues, represents a critical step towards more reproducible and comparative metabolic research in this important model organism. β€’ Keywords: Drosophila; method; mitochondria; standard; permeabilization; oxygen diffusion; probe β€’ Bioblast editor: Tindle-Solomon L β€’ O2k-Network Lab: BR Rio de Janeiro Oliveira MF

ORCID: ORCID.png Oliveira Marcus F, ORCID.png Aveiro Yan, ORCID.png Couto-Lima Carlos A

MitoFit Preprint

Β» Oliveira 2023 MitoFit Spotlight


Labels: MiParea: Respiration 


Organism: Drosophila 

Preparation: Permeabilized tissue, Homogenate 




BEC 

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