Oroboros O2k

Oroboros O2k

MitoPedia O2k and high-resolution respirometry: Oroboros Open Support

Description

The Oroboros O2k, the modular system for high-resolution respirometry (HRR), is one of the models of the Oroboros Bioenergetics Platform for mitochondria and cell research, unique for precision OXPHOS analysis. The O2k provides the basis for HRR applications combined with measurement of ROS production, mitochondrial membrane potential, ATP production, Ca²⁺, pH, and PhotoBiology. The O2k is distinguished from the NextGen-O2k.

The Oroboros allows bioenergetic analysis at controlled oxygen levels. Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells, to living cells and tissues slices.

The globally tested and trusted high-resolution Oroboros prioritizes both quality and scientific research output in the field of mitochondrial physiology and pathology, extended to PhotoBiology. The Oroboros is a robust sole-source instrument with a long life time and low running costs. Expert service is provided by the Oroboros Open Support.

» Marketplace: Oroboros

» The O2k-Series J supports the Oroboros PhotoBiology Module

Abbreviation: O2k

Reference: Purchase information, Oroboros-Top 10

» Oroboros O2k-Series J Manual

What is an O2k and the Oroboros?

O2k: The Oroboros measures oxygen (dioxygen, O₂) as a gas dissolved in aqueous solution.

With the Oroboros O2k and Oroboros NextGen-O2k, experimental data are displayed not only of oxygen concentration over time; but real-time transformation of these data allows the experimenter to follow respiration (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is running.
An Oxygraph-2k (Oroboros) is used for high-resolution monitoring of respiratory activity of biological or biochemical samples transferred into the experimental chamber. The Oroboros is not used to measure oxygen concentration in a tissue.

O2k: The Oroboros O2k is the 2^nd generation of our high-resolution respirometers. The first model was available as of 1992. Development of the new O2k started in 2k (2000) and the first O2k was delivered in 2002. The NextGen-O2k is available since 2022.

O2k: The O2k and NextGen-O2k has two experimental chambers (k: Kammern, German for chambers). Why 0.5 to 2 mL?

Power-O2k: Several Oroboros Bioenergetics Platforms are operated simultaneously (P1, P2, ..) to combine high-resolution respirometry with high-output.