Salicylhydroxamic acid

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Salicylhydroxamic acid


Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.

Abbreviation: SHAM

Reference: Parrino_2000_J_Exp_Biol

Application in HRR

SHAM: Salicylhydroxamic acid (2-Hydroxybenzohydroxamic acid; HOC6H4CONHOH), Sigma-Aldrich: S607, store at RT, CAS: 89-73-6, M = 153.14 gΒ·mol-1
Hazard statements: H312, H332, H315, H319, H335, H351; harmful in contact with skin, harmful if inhaled, causes skin irritation, causes serious eye irritation, may cause respiratory irritation, suspected of causing cancer
SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.

Preparation of 250 mM stock solution (dissolved in DMSO):
  1. Weigh 76.57 mg of SHAM.
  2. Dissolve in 1950 Β΅L of DMSO.
  3. Divide into 0.2 mL portions
  4. Store at -20 Β°C.
Β» O2k Manual Titrations: MiPNet09.12 O2k-Titrations
  • Titration volume: 8 Β΅L
  • Final conc. in 2 mL O2k chamber: 1 mM


MitoPedia topics: Inhibitor 

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