Schmitt 2023 BPS2023 San Diego

From Bioblast
Erich Gnaiger
Schmitt S, Cardoso LHD, Cecatto C, Komlodi T, Gnaiger Erich (2023) Mitochondrial bioenergetics – a quantitative analytical and diagnostic approach. 67th Annual Meeting of the Biophysical Society.

Link: Biophysical society annual meeting

Schmitt Sabine, Cardoso Luiza HD, Cecatto Cristiane, Komlodi Timea, Gnaiger Erich (2023)

Event: BPS2023 San Diego US

Mitochondrial fitness is essential for brain and muscle function, for resistance against preventable and age-related degenerative diseases and therefore for quality of life. The capacity of oxidative phosphorylation (OXPHOS) is a fundamental part of mitochondrial fitness and a key element in bioenergetics. Comprehensive and real-time OXPHOS analysis is based on biophysical and biochemical concepts related to mitochondrial core energy metabolism. It extends bioenergetics to the level of mitochondrial physiology for functional diagnosis in health and disease.

The Oroboros O2k is the state-of-the art respirometer for quantitative high-resolution respirometry (HRR) and comprehensive OXPHOS analysis. It has the high signal stability and unrestricted flexibility of titrations suited for application of complex substrate-uncoupler-inhibitor titration (SUIT) protocols which are the basis for investigating mitochondrial pathways and respiratory control in health and disease. High resolution and precise control of oxygen concentration enable studies of mitochondrial functions under normoxia, hypoxia, and hyperoxia.

With the O2k-FluoRespirometer, fluorometric assays for ROS production, mitochondrial membrane potential, ATP production, and calcium uptake can be measured in real-time and simultaneously in direct conjunction with HRR. Modules for monitoring Q- and NAD-redox states and PhotoBiology are implemented in the NextGen-O2k, further extending the analytical resolution and opening new windows to study biophysical principles of bioenergetics.

We will present applications of the NextGen-O2k and O2k-FluoRespirometer to explore mitochondrial physiology and pathology in various types of samples and find solutions to mitochondria-associated diseases.

β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit

Affiliations and support

Sabine Schmitt1, Luiza HD Cardoso1, Cristiane Cecatto1, Timea KomlΓ³di1,2, Erich Gnaiger1*
  1. Oroboros Instruments, Innsbruck, Austria.
  2. Department of Biochemistry Semmelweis University, Budapest, Hungary
* Presenting author

List of abbreviations, terms and definitions - MitoPedia

Β» MitoPedia: Terms and abbreviations

Labels: MiParea: Respiration, Instruments;methods 

HRR: Oxygraph-2k, O2k-Fluorometer 

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