TPP+ inhibitory effect

high-resolution terminology - matching measurements at high-resolution

TPP+ inhibitory effect

Description

A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP⁺ inhibitory effect (this also applies to TPMP⁺ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP⁺, and for restriction of experimental TPP⁺ concentrations below the inhibitory range.

Reference: MiPNet14.05

Bioblast links: Force and membrane potential - >>>>>> - Click on [Expand] or [Collapse] - >>>>>>

Fundamental relationships

» Force

» Affinity

» Flux

» Advancement

» Advancement per volume

» Stoichiometric number

mt-Membrane potential and protonmotive force

» Protonmotive force

» Mitochondrial membrane potential

» Chemical potential

» Faraday constant

» Format

» Uncoupler

Oroboros Potentiometry

» TPP ISE-Module

» Oroboros Manual: MiPNet15.03 O2k-MultiSensor-ISE

» TPP - Oroboros Procedures: Tetraphenylphosphonium

» Specifications: MiPNet15.08 TPP electrode

» Poster

» Unspecific binding of TPP+

» TPP+ inhibitory effect

Oroboros FluoRespirometry

» Oroboros Catalogue: Oroboros FluoRespirometer

» Oroboros Manual: MiPNet22.11 O2k-FluoRespirometer manual

» Safranin - Oroboros Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin

» TMRM - Oroboros Procedures: TMRM

Oroboros Publications

» O2k-Publications: mt-Membrane potential

» O2k-Publications: Coupling efficiency;uncoupling

MitoPedia methods: Respirometry 

MitoPedia topics: Inhibitor