Antimycin A: Difference between revisions
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'''Ama: Antimycin A''' (Antimycin A from ''Streptomyces'' sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540) | '''Ama: Antimycin A''' (Antimycin A from ''Streptomyces'' sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540) | ||
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | <span style="color:#8B008B"> '''Caution:''' '''Very toxic!!! ''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol): | '''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol): | ||
::1) weigh 5. | ::1) weigh 5.4 mg of Antimycin A into a small glass vial !Difficult to weigh - appears to be hygroscopic! | ||
::2) add 2 ml Ethanol | ::2) add 2 ml Ethanol | ||
::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | ::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | ||
Revision as of 13:27, 3 December 2010
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
[[Description::Inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor)[1].]]
Abbreviation: Ama
Reference: [[Info::MiPNet09.12,]]
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity
HRR: Chemicals; Media
Application in HRR
Ama: Antimycin A (Antimycin A from Streptomyces sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540)
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 5.4 mg of Antimycin A into a small glass vial !Difficult to weigh - appears to be hygroscopic!
- 2) add 2 ml Ethanol
- 3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2.5 µM
