Azide: Difference between revisions
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{{MitoPedia | |||
|abbr=Azd | |||
|description='''Sodium azide''' is an inhbitor of [[Complex IV|cytochrome c oxidase]] (COX, CcO). | |||
|info=[[MiPNet03.02]] | |||
|type=Inhibitor ETS | |||
}} | |||
{{MitoPedia methods|type=Inhibitor ETS | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
|type=Inhibitor ETS | |||
}}== Application in [[HRR]] == | |||
'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; FW = 65.01. | |||
<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | |||
'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>0): | |||
::1) Weigh 260 mg of sodium azide. | |||
::2) Dissolve in 1 ml H<sub>2</sub>0. | |||
::3) Divide into 0.5 ml portions. | |||
::4) Store at -20 ยฐC. | |||
'''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::* Titration volume: โฅ50 ยตl using a 50 ยตl syringe (2 ml O2k-chamber). | |||
::* Final concentration: โฅ100 mM. |
Revision as of 12:05, 6 August 2019
Description
Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).
Abbreviation: Azd
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.
Caution: Very toxic!
Preparation of 4 M storage and stock solution (dissolved in H20):
- 1) Weigh 260 mg of sodium azide.
- 2) Dissolve in 1 ml H20.
- 3) Divide into 0.5 ml portions.
- 4) Store at -20 ยฐC.
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: โฅ50 ยตl using a 50 ยตl syringe (2 ml O2k-chamber).
- Final concentration: โฅ100 mM.