Talk:ADP: Difference between revisions
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[[Image:BB-Bioblast.jpg|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]] | [[Image:BB-Bioblast.jpg|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]] | ||
== Popular Bioblast page == | == Popular Bioblast page == | ||
[[ADP]] (D) has been accessed more than | ::: [[ADP]] (D) has been accessed more than | ||
:* 15,000 times (2015-10-21) | ::::* 35,000 times (2019-12-11) | ||
:* 10,000 times (2014-11-03) | ::::* 30,000 times (2019-07-22) | ||
:*Β 5,000 times (2012-10-12) | ::::* 25,000 times (2019-03-13) | ||
::::* 20,000 times (2016-11-06) | |||
::::* 15,000 times (2015-10-21) | |||
::::* 10,000 times (2014-11-03) | |||
::::*Β 5,000 times (2012-10-12) | |||
== Former Instructions == | |||
Β | |||
These preparation instructions are still correct. Ordering ADP in portions of 1 g per vial turned out to be very useful and is therefore the recommended strategy (as described on the ADP main page). | |||
Β | |||
=== Preparation without MgCl<sub>2</sub> and lower amounts of ADP === | |||
:::: '''Preparation of 500 mM ADP stock solution''' (dissolved in H<sub>2</sub>O): | |||
Β | |||
::::# Weigh 501.3 mg of ADP = 1 mmol ADP | |||
::::# Add 1.2 mL H<sub>2</sub>OΒ (ADP is not dissolved at this stage). | |||
::::# Neutralize with 5 M KOH (approx. 450 Β΅L). ADP will dissolve after addition of KOH. | |||
::::# Check pH and adjust to 7 with 5 M KOH if necessary. | |||
::::# Adjust final volume to 2 mL and divide into 0.2 mL portions. | |||
::::# Store at -80 Β°C. | |||
=== Preparation with MgCl<sub>2</sub> and lower amounts of ADP === | |||
Β | |||
:::: '''PreparationΒ of 500 mM ADP stock solution with 300 mM freeΒ Mg<sup>2+</sup>''' (dissolved inΒ H<sub>2</sub>O): | |||
Β | |||
::::# Weigh 501.3 mg of ADP = 1 mmol ADP. | |||
::::# Add 1.2 mL H<sub>2</sub>OΒ (ADP is not dissolved at this stage). | |||
::::# Neutralize with 5 M KOH (approx. 450 Β΅l). ADP will dissolve after addition of KOH. | |||
::::# Add 121.98 mg MgCl<sub>2</sub>. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT. | |||
::::# Check pH and adjust to 7 with 5 M KOH if necessary. | |||
::::# Adjust final volume to 2 mL and divide into 0.2 mL portions. | |||
::::# Store at -80 Β°C. |
Latest revision as of 12:42, 24 February 2021
Popular Bioblast page
- ADP (D) has been accessed more than
- 35,000 times (2019-12-11)
- 30,000 times (2019-07-22)
- 25,000 times (2019-03-13)
- 20,000 times (2016-11-06)
- 15,000 times (2015-10-21)
- 10,000 times (2014-11-03)
- 5,000 times (2012-10-12)
- ADP (D) has been accessed more than
Former Instructions
These preparation instructions are still correct. Ordering ADP in portions of 1 g per vial turned out to be very useful and is therefore the recommended strategy (as described on the ADP main page).
Preparation without MgCl2 and lower amounts of ADP
- Preparation of 500 mM ADP stock solution (dissolved in H2O):
- Weigh 501.3 mg of ADP = 1 mmol ADP
- Add 1.2 mL H2O (ADP is not dissolved at this stage).
- Neutralize with 5 M KOH (approx. 450 Β΅L). ADP will dissolve after addition of KOH.
- Check pH and adjust to 7 with 5 M KOH if necessary.
- Adjust final volume to 2 mL and divide into 0.2 mL portions.
- Store at -80 Β°C.
Preparation with MgCl2 and lower amounts of ADP
- Preparation of 500 mM ADP stock solution with 300 mM free Mg2+ (dissolved in H2O):
- Weigh 501.3 mg of ADP = 1 mmol ADP.
- Add 1.2 mL H2O (ADP is not dissolved at this stage).
- Neutralize with 5 M KOH (approx. 450 Β΅l). ADP will dissolve after addition of KOH.
- Add 121.98 mg MgCl2. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT.
- Check pH and adjust to 7 with 5 M KOH if necessary.
- Adjust final volume to 2 mL and divide into 0.2 mL portions.
- Store at -80 Β°C.