Talk:Pyruvate: Difference between revisions

Latest revision as of 13:15, 8 March 2021

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10,000 times (2019-08-07)
5,000 times (2016-03-21)

Former Instructions

These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.

Preparation of 2 M stock solution (dissolved in H₂O) for use in 2-mL O2k-chamber:

Prepare fresh everyday.
Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
Add 0.2 mL H₂O.
Adjust pH to 7.0.

Preparation of 2.5 M stock solution (dissolved in H₂O) for use in 0.5-mL O2k-chamber:

Prepare fresh everyday.
Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
Add 0.2 mL H₂O.
Adjust pH to 7.0.

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 ::::*  10,000 times (2019-08-07)
 ::::*  5,000 times (2016-03-21)
+== Former Instructions ==
-== Application in [[HRR]] (updated instructions) ==
+These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
-::: '''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; M = 110.0 g·mol<sup>-1</sup>
+:::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)  for use in '''2-mL O2k-chamber''':
-:::: It is possible to weigh the powder beforehand in the Eppendorf-type tubes and store these tubes at 4 °C, to be diluted only on the day of use.
-:::: 2021-02: The preparation instructions were updated to take the volume of the solute (P) into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
-:::: '''Preparation of 2 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O)  for use in '''2-mL O2k-chamber''':
 ::::# Prepare fresh everyday.
 ::::# Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
-::::# Add 180 µL H<sub>2</sub>O.
+::::# Add 0.2 mL H<sub>2</sub>O.
 ::::# Adjust pH to 7.0.
-:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+:::: '''Preparation of 2.5 M stock solution''' (dissolved in H<sub>2</sub>O)  for use in '''0.5-mL O2k-chamber''':
-::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
-::::* Final concentration: 5 mM.
-:::: '''Preparation of 2.5 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O)  for use in '''0.5-mL O2k-chamber''':
 ::::# Prepare fresh everyday.
 ::::# Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
-::::# Add 175 µL H<sub>2</sub>O.
+::::# Add 0.2 mL H<sub>2</sub>O.
 ::::# Adjust pH to 7.0.
-:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
-::::* Final concentration: 5 mM.