Malate: Difference between revisions
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'''M: Malate''' (L-Malic acid, C<sub>4</sub>H<sub>6</sub>0<sub>5</sub>); Sigma M 1000, 100 g, store at R.T.; FW = 134.1 | '''M: Malate''' (L-Malic acid, C<sub>4</sub>H<sub>6</sub>0<sub>5</sub>); Sigma M 1000, 100 g, store at R.T.; FW = 134.1 | ||
'''Preparation of | '''Preparation of 400 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) | ::1) Weigh 268.2 mg of L-Malic acid ย | ||
::2) | ::2) Add 3 ml H<sub>2</sub>Oย ย | ||
::3) | ::3) Neutralize with 10 N KOH (approx. 750 ยตl) | ||
::4) | ::4) Adjust final volume to 5 ml (in 5 mL volumetric glass flask) | ||
::5) | ::5) Divide into 0.5 ml portions ย | ||
::6) | ::6) Store frozen at -20 ยฐC | ||
Comment: 800 mM stock until 2013-11-20 | |||
'''Oxygraph-2k Manual Titrations:'''ย [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:'''ย [[MiPNet09.12]] |
Revision as of 16:47, 20 November 2013
Description
Malate and glutamate are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations as mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. Malate dehydrogenase located in the mitochondrial matrix oxidizes malate to oxaloacetate.
Abbreviation: M
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12
MitoPedia topics: Substrate and metabolite
Application in HRR
M: Malate (L-Malic acid, C4H605); Sigma M 1000, 100 g, store at R.T.; FW = 134.1
Preparation of 400 mM stock solution (dissolved in H2O):
- 1) Weigh 268.2 mg of L-Malic acid
- 2) Add 3 ml H2O
- 3) Neutralize with 10 N KOH (approx. 750 ยตl)
- 4) Adjust final volume to 5 ml (in 5 mL volumetric glass flask)
- 5) Divide into 0.5 ml portions
- 6) Store frozen at -20 ยฐC
Comment: 800 mM stock until 2013-11-20
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 ยตl using a 25 ยตl Hamilton syringe
- Final conc. in 2 ml O2k-chamber: 2 mM