Coupling-control ratio: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''CCR'' | |abbr=''CCR'' | ||
|description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E|''R/E'']]. | |description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']]. | ||
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]], [[Pesta 2012 Methods Mol Biol]] | |info=[[Gnaiger 2009 Int J Biochem Cell Biol]], [[Pesta 2012 Methods Mol Biol]] | ||
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== Biochemical coupling efficiency: from 0 to <1 == | == Biochemical coupling efficiency: from 0 to <1 == | ||
* ''More details:'' ยป[[ | * ''More details:'' ยป[[ETS coupling efficiency]] |
Revision as of 00:34, 4 September 2014
Description
Coupling control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial substrate state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, Jref, is defined by taking Jref as the maximum flux, i.e. flux in the ETS state, E, such that the lower and upper limits of CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E.
Abbreviation: CCR
Reference: Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
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Biochemical coupling efficiency: from 0 to <1
- More details: ยปETS coupling efficiency