Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 08:52, 27 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

Next Week

2024 Week 23

Jun-03 Mo

Jun-04 Tu

Jun-05 We

Jun-06 Th

"Silencing of mt-tRF-Leu^TAA in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."

Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. »Bioblast link«

from the O2k-Network »CH Lausanne Place N«

Jun-07 Fr

@@ Line 1: / Line 1: @@
-* '''May-04 Mo'''
+{{Template:Science for Ukraine}}
+Week 22
+* '''May-27 Mo'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
+----
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
+----
+* '''May-30 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-Perform and apply the instrumental background test:<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
+----
+* '''May-31 Fr'''
+----
+'''<big>Next Week</big>'''
+Week 23
+* '''Jun-03 Mo'''
 ----
-* '''May-05 Tu'''
+* '''Jun-04 Tu'''
-::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''
-::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«  »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«
-::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. - [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
 ----
-* '''May-06 We'''
+* '''Jun-05 We'''
 ----
-* '''May-07 Th'''
+* '''Jun-06 Th'''
 :{|
-| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: »'''[[Malate anaplerotic pathway control state]]'''«
+|'''"Silencing of mt-tRF-Leu<sup>TAA</sup> in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."''' <br>
+* Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. [[Jacovetti 2024 Mol Metab|»Bioblast link«]]
-- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«
+:from the O2k-Network »[[CH Lausanne Place N]]«
-- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »'''[[SUIT-002 |SUIT-002]]'''«
 |}
-<br />
 ----
-* '''May-08 Fr'''
+* '''Jun-07 Fr'''
+----