Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 08:52, 27 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

Next Week

2024 Week 23

Jun-03 Mo

Jun-04 Tu

Jun-05 We

Jun-06 Th

"Silencing of mt-tRF-Leu^TAA in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."

Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. »Bioblast link«

from the O2k-Network »CH Lausanne Place N«

Jun-07 Fr

@@ Line 1: / Line 1: @@
-Week 42
+{{Template:Science for Ukraine}}
-* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
+Week 22
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select  “AmR calibration”  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
+<br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Oct-13 Tu'''
+* '''May-28 Tu'''
-:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>«
+----
+* '''May-29 We'''
 ----
-* '''Oct-14 We'''
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-:from the O2k-Network »[[US VA Richmond Lesnefsky EJ]]« <br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|»Bioblast link«]]
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Oct-15 Th'''
+* '''May-31 Fr'''
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?  '''<br>
-The O2k-sV-Module  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.   <br>
-- »'''[[O2k-sV-Module]]'''«  <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
-|}
 ----
-* '''Oct-16 Fr'''
+'''<big>Next Week</big>'''
+Week 23
+* '''Jun-03 Mo'''
+----
+* '''Jun-04 Tu'''
+----
+* '''Jun-05 We'''
+----
+* '''Jun-06 Th'''
 :{|
-| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-| '''Which SUIT protocol can you use to evaluate mitochondrial respiration of [[PBMC]] and [[Platelet|platelets]]?'''<br>
+|'''"Silencing of mt-tRF-Leu<sup>TAA</sup> in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation."''' <br>
-The following SUIT protocols will guide you through the titration experiments for these cell models <br>
+* Jacovetti C, Donnelly C, Menoud V, Suleiman M, Cosentino C, Sobel J, Wu K, Bouzakri K, Marchetti P, Guay C, Kayser B, Regazzi R (2024) The mitochondrial tRNA-derived fragment, mt-tRF-LeuTAA, couples mitochondrial metabolism to insulin secretion. [[Jacovetti 2024 Mol Metab|»Bioblast link«]]
-- »'''[[SUIT-001 O2 ce-pce D004]]'''« and »'''[[SUIT-002 O2 ce-pce D007a]]'''« <br>
+:from the O2k-Network »[[CH Lausanne Place N]]«
-- communicated by »[[Cecatto Cristiane]]« and »[[Garcia-Souza Luiz F]]«
 |}
 ----
-* '''Oct-17 Sa'''
+* '''Jun-07 Fr'''
 ----
-* '''Oct-18 Su'''
-:::: »<big>'''[[ISCaM 2020 Virtual Event]]'''</big>«