Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
+{{Template:Science for Ukraine}}
-Week 38
+Week 21
-*'''Sep-20 Mo'''
+* '''May-20 Mo'''
 :{|
-| width="100" align="center" | [[Image:MitoGlobal.jpg|45px|link=https://www.mitoglobal.org/index.php/MitoGlobal_Events|MitoGlobal Events]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-| '''World Mitochondrial Disease Week''' – Sep 19-25<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-World Mitochondrial Disease Week raises awareness of mitochondrial disease through educational, fundraising and advocacy activities.<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-See what is happening near you - »https://mitochondrialdiseaseweek.org/events/
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
+----
+* '''May-21 Tu'''
+----
+* '''May-22 We''' - International Day for Biological Diversity
+:{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-*'''Sep-21 Tu'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
+----
+'''<big>Next Week</big>'''
+Week 22
+* '''May-27 Mo'''
 :{|
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Simultaneous measurement of O<sub>2</sub> flux and mt-membrane potential: Detecting deficiencies in the mitochondrial electron transfer system''' <br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-: from the O2k-Network »[[AT Innsbruck Oroboros]]«<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.   [[Krumschnabel 2014 Methods Enzymol|»Bioblast link«]]
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Sep-22 We'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
+----
+* '''May-30 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' New excel templates for mitochondrial membrane potential analysis using fluorescence dyes'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-- »'''[[Mitochondrial_membrane_potential#Excel_analysis_templates|mt-membrane potential Excel templates]]'''« and »'''[[MiPNet24.09 General Template for Mt-membrane Potential Analysis]]'''«<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- communicated by »[[Komlodi Timea]]« and »[[Cardoso Luiza]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-*'''Sep-23 Th'''
+* '''May-31 Fr'''
-:::: [[File:SHVM.png|100px|link=https://wiki.oroboros.at/index.php/SHVM_2021_Virtual]] <big>'''[[SHVM 2021 Virtual]]'''</big> Sep 23-24
-:::: '''18:45 (GMT+2): [[Gnaiger Erich]] presenting at [[SHVM 2021 Virtual]]''': ''Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics.''
 ----
-*'''Sep-24 Fr'''