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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 09
-* '''Feb-26 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' ATP synthase = phosphorylation system? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-No, the phosphorylation system consists of adenine nucleotide translocase, phosphate carrier, and ATP synthase, which carry out the steps in the phosphorylation pathway.  <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[[Phosphorylation pathway]]'''«, »'''[https://www.bioblast.at/index.php/Gnaiger_2020_BEC_MitoPathways Mitochondrial Pathways: Chapter 2.2]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Feb-27 Tu'''
+* '''May-21 Tu'''
 ----
-* '''Feb-28 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
-* '''Feb-29 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Bed rest results in a decrease in skeletal muscle mitochondrial respiratory capacity, mainly as a result of an observed decrease in mitochondrial content"''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Noone J, Damiot A, Kenny H, Chery I, Zahariev A, Normand S, Crampes F, de Glisezinski I, Rochfort KD, Laurens C, Bareille MP, Simon C, Bergouignan A, Blanc S, O'Gorman DJ (2023) The impact of 60 days of -6° head down tilt bed rest on mitochondrial content, respiration and regulators of mitochondrial dynamics. [[Noone 2023 J Physiol|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Networks »[[US FL Orlando Goodpaster BH]]« , »[[FR Strasbourg Blanc S]]« , »[[IE Dublin O Gorman D]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-* '''Mar-01 Fr'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 10
+Week 22
-* '''Mar-04 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Do you observe an unstable O<sub>2</sub> slope negative following TIP2k titrations during an instrumental O<sub>2</sub> background test?  '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Incorrect placement of TIP2k Filter Papers can lead to liquid accumulation and convective oxygen backdiffusion. Check for proper placement by confirming they are fully in contact with the stopper receptacle.
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
 <br>
-- »'''[[MiPNet14.06_Instrumental_O2_background|Instrumental O<sub>2</sub> background]]'''«, »'''[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable O<sub>2</sub> slope negative]'''«<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
-- communicated by »[[Grings Mateus]]« and »[[Baglivo Eleonora]]«
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Mar-05 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Mar-06 We'''
+* '''May-29 We'''
 ----
-* '''Mar-07 Th'''
+* '''May-30 Th'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
+|}
 ----
-* '''Mar-08 Fr'''
+* '''May-31 Fr'''
 ----