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Latest revision as of 09:37, 21 May 2024

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2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 10
-* '''Mar-04 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Do you observe an unstable O<sub>2</sub> slope negative following TIP2k titrations during an instrumental O<sub>2</sub> background test?  '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Incorrect placement of TIP2k Filter Papers can lead to liquid accumulation and convective oxygen backdiffusion. Check for proper placement by confirming they are fully in contact with the stopper receptacle.
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- »'''[[MiPNet14.06_Instrumental_O2_background|Instrumental O<sub>2</sub> background]]'''«, »'''[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable O<sub>2</sub> slope negative]'''«<br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
-- communicated by »[[Grings Mateus]]« and »[[Baglivo Eleonora]]«
+|}
-|}
 ----
-* '''Mar-05 Tu'''
+* '''May-21 Tu'''
 ----
-* '''Mar-06 We'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
-| width="100" align="center" |[[File:BEC2024.1.jpg|right|150px|link=https://doi.org/10.26124/bec:2024-0001 |Gross 2024 BEC]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''The tale of two “best-friends,” proteins BID & MTCH2, pivotal regulators of the apoptosis and metabolism programs at the mitochondria.''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Gross A, (2024) A BID-MTCH2 love story: Energizing mitochondria until apoptosis do them part? Bioenerg Commun 2024.1. https://doi.org/10.26124/bec:2024-0001
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-* '''Mar-07 Th'''
+* '''May-23 Th'''
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' Which medium can be used for the instrumental background test?'''<br>
-When using dithionite, the instrumental background test should be performed in mitochondrial respiration medium MiR05, MiR05-Kit or MiR06; or alternatively, in a strong alkaline phosphate buffer (>100 mM; >pH 8), to avoid side reactions that might interfere with the test.<br>
-- »'''[[MiPNet14.06_Instrumental_O2_background]]'''«<br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-|}
 ----
-* '''Mar-08 Fr'''
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 11
+Week 22
-* '''Mar-11 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered.   <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Mar-12 Tu'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
 ----
-* '''Mar-13 We'''
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:BEC-logo.png|100px|link=https://www.bioenergetics-communications.org/index.php/bec |Bioenergetics Communications]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''''Rewarded'' Open Access - compensating authors'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-Together with the MiP''society'', BEC is raising funds to reward the first 10 papers accepted for publication in BEC in 2024: 600 EUR to attend EBEC2024 and 2-year MiP''membership''.
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
--»[https://whydonate.com/en/fundraising/BEC Support open access in science]«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- »[https://www.bioenergetics-communications.org/index.php/bec/about/submissions Submit a manuscript]«
 |}
 ----
-* '''Mar-14 Th'''
+* '''May-31 Fr'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Mitochondrial ultrastructure and respiration are affected during EDL [''Extensor digitorum longus'' muscle] aging.''' <br>
-* Fernando R, Shindyapina AV, Ost M, Santesmasses D, Hu Y, Tyshkovskiy A, Yim SH, Weiss J, Gladyshev VN, Grune T, Castro JP (2023) Downregulation of mitochondrial metabolism is a driver for fast skeletal muscle loss during mouse aging.  [[Fernando 2023 Commun Biol|»Bioblast link«]]
-:from the O2k-Network »[[DE Leipzip Ost M]]«
-|}
-----
-* '''Mar-15 Fr'''
 ----