Chang 2015 Abstract EB2015: Difference between revisions
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|area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style | |area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style | ||
|organism=Human | |organism=Human | ||
| | |tissues=Macrophage-derived | ||
|preparations=Intact cells, Permeabilized cells | |preparations=Intact cells, Permeabilized cells | ||
|pathways=F, N, NS | |pathways=F, N, NS |
Revision as of 16:09, 9 November 2016
Different effects of interval and continuous exercise regimens on capacity of mitochondria oxidative phosphorylation in lymphocyte. |
Link: Poster
Event: EB2015
Mitochondrial biogenesis is a critical metabolic adaptation to aerobic exercise training. What kind of the exercise strategy that enhances mitochondria oxidative phosphorylation in lymphocyte and subsequently improves immune function has not yet been established. This study elucidates how interval and continuous exercise regimens affect capacity of mitochondria oxidative phosphorylation in lymphocytes.
Twenty-four sedentary males were randomized to perform either aerobic interval training (AIT; 3-minute intervals at 40% and 80%VO2max, n=12) or moderate continuous training (MCT; sustained 60%VO2max, n=12) for 30 minutes/day, 5 days/week for 6 weeks. According to a novelistically designed Substrate-Uncoupler-Inhibitor Titrations (SUIT) protocol, various modes of mitochondrial respiratory control were analyzed by a high resolution respirometer (OROBOROS Oxygraph-2k).
The results demonstrated that both AIT and MCT regimens significantly increased ATP-linked O2 consumption rate (OCR), elevated reserve capacity of OCR, and lowered non-mitochondrial OCR in intact lymphocytes. Moreover, AIT modestly enhanced pyruvate plus glutamate-mediated OCR, whereas MCT elicited predominant succinate- and palmitoyl carnitine-mediated OCR in permeabilized lymphocytes.
Either AIT or MCT effectively enhances capacity of mitochondria oxidative phosphorylation in lymphocytes. Furthermore, AIT has a comprehensive improvement in mitochondria Complex I-linked respiration, while MCT may shift mitochondrial energy transduction into Complex II- and electron transferring flavoprotein-linked respiration.
• O2k-Network Lab: TW Taoyuan Wang JS
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Macrophage-derived
Preparation: Intact cells, Permeabilized cells
Pathway: F, N, NS HRR: Oxygraph-2k
Affiliations
Graduate Inst Rehabilitation Sc, Chang Gung Univ, Tao-Yuan, Taiwan