Fluorescence-Sensor: Difference between revisions
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== O2k- | == O2k-Guide == | ||
=== Select the Fluorescence-Sensors === | === Select the Fluorescence-Sensors === | ||
Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters: | Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters: | ||
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# [[Fluorescence-Sensor Cyan|LED 480 nm wavelength: Cyan]]. The installed Filter-Cap is ready for measurements with Magnesium greenยฎ or Calcium greenยฎ. A different filter is used for measurement of mt-membrane potential with safranin. | # [[Fluorescence-Sensor Cyan|LED 480 nm wavelength: Cyan]]. The installed Filter-Cap is ready for measurements with Magnesium greenยฎ or Calcium greenยฎ. A different filter is used for measurement of mt-membrane potential with safranin. | ||
ย '''Setup - next step | ย '''O2k-Guide: Setup - next step [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]''' | ||
[[File:Connect Fluorescence-Sensor lettered.JPG|400px|right|link=]] | [[File:Connect Fluorescence-Sensor lettered.JPG|400px|right|link=]] | ||
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Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet12.06]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers. | Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet12.06]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers. | ||
ย '''O2k- | ย '''O2k-Guide: [[O2k-Fluorescence_LED2-Module#Electronic_settings|Electronic settings]]''' | ||
<br> {{Technical service}} | <br> {{Technical service}} |
Revision as of 15:36, 14 March 2012
Fluorescence-Sensor
Description | Fluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Cyan. |
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Product ID | 442## |
Type | O2k, MultiSensor, O2k-Fluorescence LED2-Module |
Link | O2k-Fluorescence LED2-Module |
Image |
O2k-Guide
Select the Fluorescence-Sensors
Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters:
- LED 525 nm wavelength: Green. The installed Filter-Cap is ready for H2O2 measurement with Amplexยฎ UltraRed.
- LED 480 nm wavelength: Cyan. The installed Filter-Cap is ready for measurements with Magnesium greenยฎ or Calcium greenยฎ. A different filter is used for measurement of mt-membrane potential with safranin.
O2k-Guide: Setup - next step Mounting a Filter-Cap
Connect Fluorescence-Sensor to O2k
For recording of fluorescence signals, insert the black sensor into the window of the O2k chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit.
Stoppers
Use only black PEEK stoppers in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See MiPNet12.06 for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers.
O2k-Guide: Electronic settings