De Araujo 2015 Cold Spring Harb Protoc-A: Difference between revisions
(Created page with "{{Publication |title=de AraΓΊjo ME, Lamberti G, Huber LA (2015) Homogenization of Mammalian Cells. Cold Spring Harb Protoc 2015(11):pdb.prot083436. |info=http://www.ncbi.nlm.nih...") Β |
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{{Publication | {{Publication | ||
|title=de AraΓΊjo ME, Lamberti G, Huber LA (2015) | |title=de AraΓΊjo ME, Lamberti G, Huber LA (2015) Isolation of Early and Late Endosomes by Density Gradient Centrifugation. Cold Spring Harb Protoc 2015(11):pdb.prot083444. | ||
|info=http://www.ncbi.nlm.nih.gov/pubmed/ | |info=http://www.ncbi.nlm.nih.gov/pubmed/26527762 | ||
|authors=de AraΓΊjo ME, Lamberti G, Huber LA | |authors=de AraΓΊjo ME, Lamberti G, Huber LA | ||
|year=2015 | |year=2015 | ||
|journal=Cold Spring Harb Protoc | |journal=Cold Spring Harb Protoc | ||
|abstract= | |abstract=Density gradient centrifugation is a common method for separating intracellular organelles. During centrifugation, organelles float or sediment until they reach their isopycnic position within the gradient. The density of an organelle depends on its content, size, shape, and the lipid:protein ratio. The degree of separation between different organelles will therefore be highly dependent on how different their isopycnic points are in a given buffer. Separation will also depend on the medium used to prepare the gradient, whether it is sucrose (the most common) or an alternative. Here we describe the use of both continuous and discontinuous (step) gradients to isolate endocytic organelles. | ||
}} | }} | ||
{{Labeling}} | {{Labeling}} |
Revision as of 09:53, 12 January 2016
de AraΓΊjo ME, Lamberti G, Huber LA (2015) Isolation of Early and Late Endosomes by Density Gradient Centrifugation. Cold Spring Harb Protoc 2015(11):pdb.prot083444. |
Β» http://www.ncbi.nlm.nih.gov/pubmed/26527762
de AraΓΊjo ME, Lamberti G, Huber LA (2015) Cold Spring Harb Protoc
Abstract: Density gradient centrifugation is a common method for separating intracellular organelles. During centrifugation, organelles float or sediment until they reach their isopycnic position within the gradient. The density of an organelle depends on its content, size, shape, and the lipid:protein ratio. The degree of separation between different organelles will therefore be highly dependent on how different their isopycnic points are in a given buffer. Separation will also depend on the medium used to prepare the gradient, whether it is sucrose (the most common) or an alternative. Here we describe the use of both continuous and discontinuous (step) gradients to isolate endocytic organelles.
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