Nicotinamide adenine dinucleotide: Difference between revisions

Revision as of 05:23, 12 February 2016

high-resolution terminology - matching measurements at high-resolution

Nicotinamide adenine dinucleotide

Description

Nicotinamide adenine dinucleotide, NAD and NADH is not permeable through the inner mt-membrane. The NADH pool integrates the activity of the TCA cycle and various matrix dehydrogenases upstream of CI, and thus forms a junction or funnel of electron transfer to CI, the N-junction (compare F-junction, Q-junction). Cytosolic NADH is effectively made available for mitochondrial respiration through the malate-aspartate shuttle or glycerophosphate dehydrogenase Complex.

Abbreviation: NADH

MitoPedia topics: Substrate and metabolite

Labels:

Enzyme: Complex I

Application in HRR

Nicotinadeninedinucleotide, NADH (β-Nicotinamide adenine dinucleotide, reduced potassium salt, C₂₁H₂₇N₇O₁₄P₂K₂); Sigma A 4505, 100 mg, store at -20 °C (old Sigma recommendation: 4-8 °C); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [1]. The same precautions are recommended by Sigma for the sodium salt.

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 {{MitoPedia
-|abbr=NAD, NADH
+|abbr=NADH
+|description='''Nicotinamide adenine dinucleotide''', NAD and NADH is not permeable through the inner mt-membrane.  The NADH pool integrates the activity of the [[TCA cycle]] and various matrix dehydrogenases upstream of [[CI]], and thus forms a junction or funnel of electron transfer to CI, the [[N-junction]] (compare [[F-junction]], [[Q-junction]]). Cytosolic NADH is effectively made available for mitochondrial respiration through the [[malate-aspartate shuttle]] or [[Glycerophosphate_dehydrogenase_complex|glycerophosphate dehydrogenase Complex]].
+|type=Substrate ETS
 }}
-{{MitoPedia concepts}}
+{{MitoPedia methods|type=Substrate ETS
-{{MitoPedia methods}}
+}}
-{{MitoPedia O2k and high-resolution respirometry}}
+{{MitoPedia topics
-{{MitoPedia topics}}
+|mitopedia topic=Substrate and metabolite
+|type=Substrate ETS
+}}
+{{Labeling
+|enzymes=Complex I
+}}
+== Application in [[HRR]]  ==
+'''Nicotinadeninedinucleotide, NADH''' (β-Nicotinamide adenine dinucleotide, reduced potassium salt, C<sub>21</sub>H<sub>27</sub>N<sub>7</sub>O<sub>14</sub>P<sub>2</sub>K<sub>2</sub>);  Sigma A 4505, 100 mg, store at -20 °C (old Sigma recommendation: 4-8 °C); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n4505pis.pdf]. The same precautions are recommended by Sigma for the sodium salt.