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O2k-Workshop on High-Resolution Respirometry - O2k-Basic and TPP-Basic

Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011.

Hotel Körbersee

More information on IOC61: http://www.oroboros.at/index.php?ioc61

Final programme: download (2011-04-19; 933 kb)

Abstracts

Look at Abstracts >>

Participants and areas of interest

Alexeyev Mikhail, USA: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
Angelin Alessia, USA: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy).
Balboa Castillo Elisa, Chile: Increase in mitochondrial cholesterol and dysfunction in NPC.
Castelein Natascha, Belgium: The role of mitochondria in dietary restriction induced lifespan extension in C. elegans.
Gabrielova Eva, Czech Republic: Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.
Guadalupe Grau Amelia, Spain: Mitochondrial physiology in human skeletal muscle. Roles of mitochondria in obesity and exercise.
Hals Ingrid, Norway: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
Hancock Saege, USA
Kasal Daniel, Brazil: Modulation of cardiomyocyte respiration by bone marrow stem cells.
Kilic Ana, Germany: Respiration in isolated murine tissues; uncoupled respiration in mitochondria.
Kovalcikova Jana, Czech Republic : Respiration and oxphos regulation in heart mitochondrial populations under oxidative stress.
Labieniec Magdalena, Poland: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
Le Catherine, USA: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential.
Magnifico Maria Chiara, Italy: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells.
Mandl Markus, Vienna
Manjeri Ganesh, The Netherlands: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts.
Marelsson Sigurdur, Sweden: The role of mitochondrial dysfunction in encephalopathy.
Morein Torbjörn, Sweden
Nuskova Hana, Czech Republic: Mitochondrial Energetics in Health and Disease.
Sanz Alberto, Finland: Mechanism causing aging and the role of mitochondria in this process.
Scherer Alves Leonardo, USA: Mitochondrial Physiology as a force of human adaptation and evolution to different geographic niches and the relation between the environment and mitochondrial genes in human common diseases.
Watala Cezary, Poland: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
Yokota Takashi, Denmark: The role of mitochondria in chronic heart failure, diabetes, or obesity.
Zurmanova Jitka, Czech Republic: Mechanisms of regulation of OXPHOS by local ADP in muscle cells.

Tutors

Erich Gnaiger (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
Mario Fasching (OROBOROS INSTRUMENTS, AT)
Dominik Pesta (Innsbruck Medical University, AT)
Zuzana Sumbalova (OROBOROS INSTRUMENTS, AT)

Feedback/Discussion

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Photos

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@@ Line 1: / Line 1: @@
 [[File:O2k-TIP2k.jpg|right|300px|caption]]
-'''O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced'''
+'''O2k-Workshop on High-Resolution Respirometry - O2k-Basic and TPP-Basic'''
 Schröcken, Vorarlberg, Austria  26 April - 1 May, 2011.
@@ Line 6: / Line 6: @@
 Hotel Körbersee
-For some pictures, see [[IOC61_Photos|IOC61]]
+More information on IOC61: http://www.oroboros.at/index.php?ioc61
-More information on IOC61: http://www.oroboros.at/index.php?nextcourse
-'''Contact''': [[Has mailaddress::{{{mailaddress|[email protected]}}}]]
 '''Final programme:''' '''[http://www.oroboros.at/fileadmin/user_upload/O2kCourseIOC/IOC61_2011-04/IOC61_2011-04_Schroecken.pdf ''download'' (2011-04-19; 933 kb)]
 '''
-==Course structure==
-* Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
-* Hot Topics in Mitochondrial Physiology: 10+5 min
 ==Abstracts==
 *[[:IOC61_Abstracts|Look at Abstracts >>]]
-*[http://www.bioblast.at/index.php/Special:AbstractsExtension?event=IOC61 Add your Abstract >>]
-For the abstract format, see [[Author_IOC61]].
-== Combined introductory and advanced O2k-Workshop with parallel sessions==
-::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:'''
-'''A1. General [[HRR]] topics'''
-* O2k-assembly,
-* [[Polarographic oxygen sensor]] (OroboPOS) service,
-* POS calibration,
-* [[DatLab 4.3|DatLab]] software: principles and operation,
-* [[Titration-Injection-microPump]] - TIP2k.
-'''A2. Protocols in HRR'''
-* Introduction to fundamental elements of mitochondrial respiratory physiology,
-* Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
-'''A3. Hands-on experiments with the O2k'''
-* Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control,
-* Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]],
-* Preparation of [[permeabilized muscle fibre]]s,
-* HRR with permeabilized muscle fibres.
-'''A4. Presentation and interpretation of [[oxygen flux]]'''
-* Normalization of respiration per tissue or per [[mitochondrial marker]],
-* Coupling control ratios,
-* Substrate control ratios,
-* Generalized flux control ratios.
-'''A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:'''
-* Mitochondrial membrane potential (TPP+ electrode),
-* Acidification (pH electrode),
-* Nitric oxide (amperometric),
-* Spectrophotometry (cytochrome spectra),
-* Spectrofluorimetry (Amplex red, safranin, etc.)
-::'''B: Advanced users will join in small working teams in parallel sessions, with specific topics:
-'''
-'''B1. O2k-MultiSensor and TIP2k'''
-* Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]]
-* Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
-* ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
-* pH-stat feedback control,
-'''B2. Theoretical perspectives'''
-* Advanced experimental protocols,
-* Quality control - towards [[quality assurance]];
-* ''Add your topic'':
 ==Participants and areas of interest==