SUIT-023: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|info='''A: | |info='''A: Determination of the respiration due to the cytochrome ''c'' pathway''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-023.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-023.pdf Versions] | ||
|application=O2 | |application=O2 | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ย | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce | ||
::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ||
The SUIT-023 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]] | The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the cytochrome ''c'' pathway, which is non-resistant to [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]] inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the [[alternative oxidase]] pathway (see [[SUIT-022]]). ย | ||
__TOC__ | __TOC__ | ||
ย Communicated by | ย Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09) | ||
== Specific SUIT protocols == | == Specific SUIT protocols == | ||
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*ย [[SUIT-023 O2 ce D053]] for intact microalgal cells (ce) | *ย [[SUIT-023 O2 ce D053]] for intact microalgal cells (ce) | ||
{{Template:SUIT- | {{Template:SUIT-023}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity of the cytochrome c | :::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity of the cytochrome ''c'' on cells on [[ROUTINE]]. To complement the study with the estimation of the mitochondrial respiration due to the [[alternative oxidase]] pathway, it must be carried out in parallel to [[SUIT-022]]. | ||
:::+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised. | :::+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised. | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::*[[SUIT-022 O2 ce D051]] for [[microalgal]] intact cells | |||
Revision as of 11:22, 9 April 2019
Description
Reference: A: Determination of the respiration due to the cytochrome c pathway ยปVersions
O2k-Application: O2
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the cytochrome c pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Specific SUIT protocols
SUIT-023 O2 ce D053
- SUIT-023 O2 ce D053 for intact microalgal cells (ce)
Strengths and limitations
- SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity of the cytochrome c on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the alternative oxidase pathway, it must be carried out in parallel to SUIT-022.
- + This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 for microalgal intact cells
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry