Kagawa 1971 J Biol Chem: Difference between revisions
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* It was shown that the reconstitution of vesicular structures with functional activity required several hours. Rapid reconstitution resulted in inactive vesicles. Evidence for the formation of new vesicles from solubilized P-lipids was obtained by demonstrating inclusion of macromolecules such as 14C-labeled inulin or ferritin which could not be removed by washing. | * It was shown that the reconstitution of vesicular structures with functional activity required several hours. Rapid reconstitution resulted in inactive vesicles. Evidence for the formation of new vesicles from solubilized P-lipids was obtained by demonstrating inclusion of macromolecules such as 14C-labeled inulin or ferritin which could not be removed by washing. | ||
|keywords= | |keywords=Oxidative phosphorylation, 32Pi-ATP exchange, Enzymes, Beef heart | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism= | |organism=Mammals | ||
|tissues=Cardiac muscle | |tissues=Cardiac muscle | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria |
Revision as of 16:45, 13 February 2013
Kagawa Y, Racker E (1971) Partial resolution of the enzymes catalyzing oxidative phosphorylation XXV. Reconstitution of vesicles catalyzing 32Pi-adenosine triphosphate exchange. J Biol Chem 246: 5477-5487. |
Kagawa Y, Racker E (1971) J Biol Chem
Abstract:
- Amorphous membrane fragments depleted in P-lipids and cytochrome oxidase were isolated from bovine heart mitochondria and were reconstituted with P-lipids and coupling factors to yield vesicular structures. These vesicles catalyzed a 32PiβATP exchange and showed an induced enhancement of anilinonaphthalene sulfonate fluorescence on addition of ATP
- 32PiβATP exchange and fluorescence enhancement were abolished by uncouplers of oxidative phosphorylation and by energy transfer inhibitors. The ATPase activity was inhibited by energy transfer inhibitors, but stimulated by uncouplers or by the combined action of nigericin and valinomycin in the presence of K+. Both ATPase activity and 32PiβATP exchange were inhibited by a specific antibody against coupling factor 1.
- It was shown that the reconstitution of vesicular structures with functional activity required several hours. Rapid reconstitution resulted in inactive vesicles. Evidence for the formation of new vesicles from solubilized P-lipids was obtained by demonstrating inclusion of macromolecules such as 14C-labeled inulin or ferritin which could not be removed by washing.
β’ Keywords: Oxidative phosphorylation, 32Pi-ATP exchange, Enzymes, Beef heart
Labels:
Organism: Mammals"Mammals" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Tissue;cell: Cardiac muscle"Cardiac muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
Made history