Jaburek 2013 Int J Biochem Cell Biol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Jaburek M, Jezek J, Zelenka J, Jezek P. (2013) Antioxidant activity by a synergy of redox-sensitive mitochondrial phospholipase A2 and uncoupling protein-2 in lung and spleen. Int J Biochem Cell Biol 45: 816- | |title=Jaburek M, Jezek J, Zelenka J, Jezek P. (2013) Antioxidant activity by a synergy of redox-sensitive mitochondrial phospholipase A2 and uncoupling protein-2 in lung and spleen. Int J Biochem Cell Biol 45:816-25 | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23354121 PMID: 23354121] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23354121 PMID: 23354121] | ||
|authors=Jaburek M, Jezek J, Zelenka J, Jezek P | |authors=Jaburek M, Jezek J, Zelenka J, Jezek P | ||
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{{Labeling | {{Labeling | ||
|organism=Mouse | |organism=Mouse | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Complex II;succinate dehydrogenase, Uncoupling protein | |||
|injuries=Oxidative stress;RONS | |||
|topics=Fatty acid | |||
|substratestates=CII, ETF | |substratestates=CII, ETF | ||
| | |instruments=Oxygraph-2k | ||
}} | }} |
Revision as of 10:39, 13 February 2015
Jaburek M, Jezek J, Zelenka J, Jezek P. (2013) Antioxidant activity by a synergy of redox-sensitive mitochondrial phospholipase A2 and uncoupling protein-2 in lung and spleen. Int J Biochem Cell Biol 45:816-25 |
Jaburek M, Jezek J, Zelenka J, Jezek P (2013) Int J Biochem Cell Biol
Abstract: Mitochondrial uncoupling protein-2 (UCP2) has been suggested to participate in the attenuation of the reactive oxygen species production, but the mechanism of action and the physiological significance of UCP2 activity remain controversial. Here we tested the hypothesis that UCP2 provides feedback downregulation of oxidative stress in vivo via synergy with an H2O2-activated mitochondrial calcium-independent phospholipase A2 (mt-iPLA2). Tert-butylhydroperoxide or H2O2 induced free fatty acid release from mitochondrial membranes as detected by gas chromatography/mass spectrometry, which was inhibited by r-bromoenol lactone (r-BEL) but not by its stereoisomer s-BEL, suggesting participation of mt-iPLA2ฮณ isoform. Tert-butylhydroperoxide or H2O2 also induced increase in respiration and decrease in mitochondrial membrane potential in lung and spleen mitochondria from control but not UCP2-knockout mice. These data suggest that mt-iPLA2ฮณ-dependent release of free fatty acids promotes UCP2-dependent uncoupling. Upon such uncoupling, mitochondrial superoxide formation decreased instantly also in the s-BEL presence, but not when mt-iPLA2 was blocked by R-BEL and not in mitochondria from UCP2-knockout mice. Mt-iPLA2ฮณ was alternatively activated by H2O2 produced probably in conjunction with the electron-transferring flavoprotein:ubiquinone oxidoreductase (ETFQOR), acting in fatty acid ฮฒ-oxidation. Palmitoyl-d,l-carnitine addition to mouse lung mitochondria, respiring with succinate plus rotenone, caused a respiration increase that was sensitive to r-BEL and insensitive to s-BEL. We thus demonstrate for the first time that UCP2, functional due to fatty acids released by redox-activated mt-iPLA2ฮณ, suppresses mitochondrial superoxide production by its uncoupling action. In conclusion, H2O2-activated mt-iPLA2ฮณ and UCP2 act in concert to protect against oxidative stress. โข Keywords: Lung, Spleen, Mitochondrial uncoupling protein-2 (UCP2), Calcium-independent phospholipase A2 (mt-iPLA2), Electron-transferring flavoprotein:ubiquinone oxidoreductase
โข O2k-Network Lab: CZ Prague Jezek P
Labels:
Stress:Oxidative stress;RONS Organism: Mouse
Preparation: Isolated mitochondria Enzyme: Complex II;succinate dehydrogenase, Uncoupling protein Regulation: Fatty acid
HRR: Oxygraph-2k