From Bioblast
Description
SUIT-catg: FNS
Abbreviation: FNS_1OctM 2D 3G 4S 5Rot 6Omy 7U 8c 9Ama
Reference: Gnaiger 2015 Scand J Med Sci Sports
MitoPedia concepts:
SUIT protocol
- Subcatg: F+FN+FNS+S
- SUIT protocol pattern: diametral
- Mark names in DatLab: SUIT_FNS_GM_01
- DatLab-Excel template: in prep. SUIT_FNS_GM_01.xlsx
Step | Respiratory state | Pathway control | Pathway to Q | Comment |
---|---|---|---|---|
1OctM | OctM(L) | F | CETF | |
2D | OctM(P) | F | CETF | OXPHOS coupling efficiency in state F. |
3G | GMOct(P) | FN | FAO&CI | F/N ratio or (N-F)/N flux control factor of OXPHOS capacity. |
4S | GMSOct(P) | FNS | FAO&CI&II | Additive effect of the NS pathway combination, compared to the sum of N+S pathway fluxes measured separately, in the OXPHOS state and the presence of F. |
5Rot | S(P) | S | CII | The P/E ratio is not obtained in the FNS state, with preference given to the next steps for OXPHOS coupling efficiency. A succinate concentration of >10 mM may be required for saturating SP capacity. |
6Omy | S(L) | S | CII | OXPHOS coupling efficiency in state S, comparable to state F. |
7U | S(E) | S | CII | The complete ETS capacity in state S may not be obtained after oligomycin. If apparent P/E>1.0, this is corrected to 1.0. |
8c | S(E) | S | CII | If cytochrome c control factors = 0.0, the cytochrome c test conducted at this very late ETS state provides an additional indication of stability of the mt-preparation during the experimental assay. Application of the cytochrome c test earlier in the protocol (SUIT_NS_GM_01: 1GM 2D 3c 4S ..) is preferable, if cytochrome c control factors > 0.0 may be observed occasionally or regulary. If such cytochrome c effects are not exclusion criteria, then OXPHOS states with all substrate combinations can be compared in the presence of cytochrome c. |
9Ama | ROX | ROX | ROX may be lower in substrate states earlier in the SUIT protocol. Therefore, this ROX measurement is frequently taken as a methodological control rather than as the basis of ROX correction of mitochondrial respiration (mt). |