Talk:Pyruvate
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Application in HRR (updated instructions)
- P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 Β°C; M = 110.0 gΒ·mol-1
- It is possible to weigh the powder beforehand in the Eppendorf-type tubes and store these tubes at 4 Β°C, to be diluted only on the day of use.
- 2021-02: The preparation instructions were updated to take the volume of the solute (P) into account (see: User:Spitzer_Gudrun/Volume_of_the_solute). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
- P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 Β°C; M = 110.0 gΒ·mol-1
- Preparation of 2 M stock solution (200 Β΅L, dissolved in H2O) for use in 2-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 180 Β΅L H2O.
- Adjust pH to 7.0.
- Β» O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 Β΅L using a 25 Β΅L Hamilton syringe.
- Final concentration: 5 mM.
- Preparation of 2.5 M stock solution (200 Β΅L, dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 175 Β΅L H2O.
- Adjust pH to 7.0.
- Β» O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 Β΅L using a 10 Β΅L Hamilton syringe.
- Final concentration: 5 mM.